Human mesenchymal stem cells may be involved in keloid pathogenesis

Background  The pathogenesis of keloid is poorly understood. Although vigorous investigations have attempted to elucidate the mechanisms or causative factors of keloid, there are little data on why keloids are very intractable and recur easily in each patient. Methods  In an attempt to analyze the p...

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Bibliographic Details
Published in:International journal of dermatology 2008-11, Vol.47 (11), p.1112-1117
Main Authors: Akino, Kozo, Akita, Sadanori, Yakabe, Aya, Mineda, Takao, Hayashi, Tomayoshi, Hirano, Akiyoshi
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Blotting, Western
Cell Differentiation
Cell Migration Assays
Cell Proliferation
Coculture Techniques
Collagen - metabolism
Dermatology
Endoplasmic Reticulum, Rough - ultrastructure
Fibroblasts - metabolism
Fibroblasts - physiology
Fibroblasts - ultrastructure
Fibronectins - metabolism
Humans
Keloid - physiopathology
Male
Medical sciences
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - physiology
Mesenchymal Stromal Cells - ultrastructure
Skin - pathology
Skin involvement in other diseases. Miscellaneous. General aspects
Young Adult
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Summary:Background  The pathogenesis of keloid is poorly understood. Although vigorous investigations have attempted to elucidate the mechanisms or causative factors of keloid, there are little data on why keloids are very intractable and recur easily in each patient. Methods  In an attempt to analyze the possible interaction between human mesenchymal stem cells and keloid‐derived fibroblasts, the dual‐chamber cell‐migration assay, cell proliferation, ultrastructural morphology, and Western blot analysis were used to investigate the production of the extracellular matrices of the coculture. Results  Cell proliferation was not significantly different between keloid‐derived fibroblasts and normal dermal fibroblasts during a 4‐day observation period. There was a significant cell migration of human mesenchymal stem cells when keloid‐derived fibroblasts were placed in the bottom chamber, compared to when normal dermal fibroblasts were placed in the same way in 8‐µm diameter pore membranes (190.6 ± 51.45 and 32.0 ± 6.20 cells/field, respectively, P 
ISSN:0011-9059
1365-4632
DOI:10.1111/j.1365-4632.2008.03380.x