Vaccines and their transfection potency

Viral vaccines have been shown to contain residual host cell-DNA. There is no doubt about uptake and expression of foreign DNA in mammalian cells but the mechanism of transfection is not completely understood. It is suggested that DNA associates with several compounds and is transferred into the cel...

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Bibliographic Details
Published in:Vaccine 1999-04, Vol.17 (17), p.2113-2116
Main Authors: Gonser, S, Stalder, J, Folkers, G
Format: Article
Language:English
Subjects:
Aluminum Hydroxide
Biological and medical sciences
Calcium Phosphates
Chloramphenicol O-Acetyltransferase - biosynthesis
Chloramphenicol O-Acetyltransferase - genetics
DEAE-Dextran
Fibrosarcoma
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Humans
Microbiology
Plasmids - genetics
Residual host cell-DNA
Transfection
Tumor Cells, Cultured
Vaccine safety
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - genetics
Viral Vaccines - genetics
Virology
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Summary:Viral vaccines have been shown to contain residual host cell-DNA. There is no doubt about uptake and expression of foreign DNA in mammalian cells but the mechanism of transfection is not completely understood. It is suggested that DNA associates with several compounds and is transferred into the cell by endocytosis. In this study we estimate the potential of transfection of several original adjuvants by adding reporter plasmid DNA (pDNA) to vaccines. We used fibrosarcoma cells as an in vitro model and the results indicate that the cells are not able to express pDNA. Therefore we propose that adjuvants included in viral vaccines have no potential to transfect fibroblasts.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(99)00007-9