Customisation of AFLP analysis for cassava varietal identification

• Published in: Phytochemistry (Oxford) 1999-03, Vol.50 (6), p.919-924
• Main Authors: Wong, Hui-Lee, Yeoh, Hock-Hin, Lim, Saw-Hoon
• Format: Article
• Language: English
• Subjects: Abstract; Agronomy. Soil science and plant productions; Biological and medical sciences; DNA Fingerprinting - methods; DNA Restriction Enzymes - metabolism; DNA, Plant - chemistry; DNA, Plant - genetics; DNA, Plant - metabolism; Electrophoresis; Fundamental and applied biological sciences. Psychology; Gene Amplification; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetic Variation; Genetics and breeding of economic plants; Manihot - chemistry; Manihot - classification; Manihot - genetics; Plant material; Polymorphism, Restriction Fragment Length
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/S0031-9422(98)00628-1

Amplified fragment length polymorphism (AFLP) markers were used in the characterization of eight cassava varieties. This non-radioactive AFLP system was customized in terms of the choice of restriction enzymes used and the selection of nucleotides added to the 3′ end of primers. EcoRI/ MseI and HindIII/ MseI fragments generally gave monomorphic profiles while ApaI/ TaqI fragments produced polymorphic profiles suggesting a genome with high G+C content. It was possible to identify the eight cassava varieties used in this study using CTG as selective bases at the TaqI primer. For cassava, the AFLP system provided a higher number of loci detected per run when compared to RAPD. The reliability accompanying AFLP analysis would thus make it suitable for the characterization of cassava varieties.