Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24

Rhodococcus strain 124 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We have identified a cosmid clone from 124 genomic DNA that is able to confer the ability to convert indene to indandiol upon Rhodococcus erythropo...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1999-06, Vol.51 (6), p.786-793
Main Authors: TREADWAY, S. L, YANAGIMACHI, K. S, LANKENAU, E, LESSARD, P. A, STEPHANOPOULOS, G, SINSKEY, A. J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Chromatography, High Pressure Liquid
Cloning
Deoxyribonucleic acid
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
Gene Library
Genes, Bacterial
Genetics
Indenes - metabolism
Liquid chromatography
Microbiology
Mission oriented research
Molecular Sequence Data
Naphthalene
Oxidoreductases - genetics
Oxidoreductases - metabolism
Oxygenases - genetics
Oxygenases - metabolism
Phylogeny
Plasmids
Rhodococcus - enzymology
Rhodococcus - genetics
Rhodococcus erythropolis
Sequence Analysis, DNA
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Summary:Rhodococcus strain 124 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We have identified a cosmid clone from 124 genomic DNA that is able to confer the ability to convert indene to indandiol upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalenetype dioxygenase. DNA sequence analysis of a portion of this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase. These genes will be useful for manipulating indene bioconversion in Rhodococcus strain 124.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530051463