Effect of time post mortem on the concentration of endotoxin in rat organs: implications for sudden infant death syndrome (SIDS)

Abstract The aim of the study was to test the following hypotheses: (i) that endotoxin injected 40 min prior to death can be detected in rat organs post mortem and (ii) that endotoxin levels do not change with increasing time post mortem. Rats were injected with or without endotoxin in buffered sali...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 1999-08, Vol.25 (1-2), p.125-130
Main Authors: Sayers, Nicola M., Crawley, Barbara A., Humphries, Kevin, Drucker, David B., Oppenheim, Beryl A., Hunt, Linda P., Morris, James A., Telford, David R.
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Biological and medical sciences
Emergency and intensive care: neonates and children. Prematurity. Sudden death
Endotoxin
Endotoxins
Endotoxins - administration & dosage
Endotoxins - analysis
Heart
Humans
Infant, Newborn
Intensive care medicine
Kidney - chemistry
Kidneys
Liver
Liver - chemistry
Male
Medical sciences
Myocardium - chemistry
Organs
Postmortem
Postmortem Changes
Rat
Rats
Rats, Sprague-Dawley
Room temperature
SIDS
Spleen
Spleen - chemistry
Sudden Infant Death - etiology
Sudden infant death syndrome
Time Factors
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Summary:Abstract The aim of the study was to test the following hypotheses: (i) that endotoxin injected 40 min prior to death can be detected in rat organs post mortem and (ii) that endotoxin levels do not change with increasing time post mortem. Rats were injected with or without endotoxin in buffered saline, 40 min prior to being killed. Endotoxin levels in rat organs were assessed using a Limulus amoebocyte assay. The effect of storage time post mortem was assessed by following various storage regimes at 25°C and 8°C. Significant differences (P=
ISSN:0928-8244
1574-695X
2049-632X
DOI:10.1111/j.1574-695X.1999.tb01335.x