Distribution and patterns of connectivity of interneurons containing calbindin, calretinin, and parvalbumin in visual areas of the occipital and temporal lobes of the macaque monkey

• Published in: Journal of comparative neurology (1911) 1999-09, Vol.412 (3), p.515-526
• Main Authors: Defelipe, Javier, González-Albo, María C., Del Río, María R., Elston, Guy N.
• Format: Article
• Language: English
• Subjects: Animals; Brain Mapping; Calbindin 2; Calbindins; calcium-binding proteins; chandelier cells; cortical circuitry; double-bouquet cells; Female; Immunohistochemistry; interneurons; Interneurons - chemistry; Macaca fascicularis - anatomy & histology; Macaca fascicularis - metabolism; Macaca fascicularis - physiology; Male; Nerve Net - physiology; Nerve Tissue Proteins - analysis; Occipital Lobe - chemistry; Occipital Lobe - cytology; Occipital Lobe - physiology; Parvalbumins - analysis; Presynaptic Terminals - chemistry; S100 Calcium Binding Protein G - analysis; Temporal Lobe - chemistry; Temporal Lobe - cytology; Temporal Lobe - physiology; visual cortex; Visual Pathways - chemistry; Visual Pathways - physiology
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/(SICI)1096-9861(19990927)412:3<515::AID-CNE10>3.0.CO;2-1

Immunocytochemical techniques were used to examine the distribution of double‐bouquet cells and chandelier cells that were immunoreactive (‐ir) for the calcium‐binding proteins calbindin (CB), calretinin (CR), and parvalbumin (PV) in the primary visual area (V1), the second visual area (V2), and cytoarchitectonic area TE in the macaque monkey. Furthermore, the connections between CB‐, CR‐, and PV‐ir neurons in these visual areas were investigated at the light microscope level by using a dual‐immunocytochemical staining procedure. The most significant findings were three‐fold. First, the number and distribution of CB‐ir and CR‐ir double‐bouquet cells and PV‐ir chandelier cells differed considerably between different visual areas. In particular, the different distribution of double‐bouquet cells was illustrated dramatically at the V1/V2 border, where CB‐ir double‐bouquet axons were very few or lacking in V1 but were very numerous in V2. Furthermore, PV‐ir chandelier cell terminals were relatively sparse in V1, more frequent in V2, and most frequent in area TE. Second, the percentage of CB‐, CR‐, and PV‐ir neurons receiving multiple contacts on their somata and proximal dendrites from other calcium‐binding protein neurons varied between 22% and 85%. The highest percentage of contacts found between immunolabelled cells and multiterminals were for the combinations CR/CB (76–85%; percent of cells immunoreactive for CB that were innervated by multiterminals immunoreactive for CR), followed by the combination PV/CR (42–48%), and then by the other combinations that had similar percentages (22–32% for CR/PV; 26–37% for CB/CR; 29–42% for CR/PV). Third, differences in the relative proportions of CB, CR, and PV terminals in contact with CB‐, CR‐, and PV‐ir neurons were consistent between the different cortical areas studied. Thus, certain characteristics of intraareal circuits differ, whereas others remain similar, in different areas of the occipitotemporal visual pathway. The differences may represent regional specializations related to the different processing of visual stimuli, whereas the similarities may be attributed to general functional requisites for interneuronal circuitry. J. Comp. Neurol. 412:515–526, 1999. © 1999 Wiley‐Liss, Inc.