Is glycation of low density lipoproteins in patients with Type 2 diabetes mellitus a LDL pre-oxidative condition?

Summary Aims The study aimed to evaluate whether low density lipoprotein (LDL) in diabetic patients is more glycated and susceptible to oxidation than in non‐diabetic subjects and investigated the hypothesis that LDL glycation is associated with an increased plasma concentration of LDL– (a circulati...

Full description

Saved in:
Bibliographic Details
Published in:Diabetic medicine 1999-08, Vol.16 (8), p.663-669
Main Authors: Moro, E., Alessandrini, P., Zambon, C., Pianetti, S., Pais, M., Cazzolato, G., Bon, G. Bittolo
Format: Article
Language:English
Subjects:
Aged
Antibodies, Monoclonal
apolipoprotein B
Apolipoproteins B - blood
Biological and medical sciences
Biomarkers - blood
Cholesterol - blood
Cholesterol, HDL - blood
Cholesterol, LDL - blood
Diabetes Mellitus, Type 2 - blood
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Enzyme-Linked Immunosorbent Assay - methods
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Female
Fructosamine - blood
Glycated Hemoglobin A - analysis
glycation
Glycosylation
Humans
Lipoproteins, LDL - blood
low density lipoproteins
Male
Malondialdehyde - blood
Medical sciences
Middle Aged
oxidation
Reference Values
Regression Analysis
Triglycerides - blood
Type 2 diabetes mellitus
Vitamin E - blood
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Aims The study aimed to evaluate whether low density lipoprotein (LDL) in diabetic patients is more glycated and susceptible to oxidation than in non‐diabetic subjects and investigated the hypothesis that LDL glycation is associated with an increased plasma concentration of LDL– (a circulating electronegatively charged LDL), proposed as an index of in vivo oxidation. Methods LDL glycation was measured by a competitive enzyme immunoadsorbent assay, using a monoclonal antibody against glycated apoB in 24 Type 2 diabetic patients and 12 healthy controls. LDL– was separated by ion‐exchange HPLC in LDL samples obtained after sequential preparative ultracentrifugation (density range 1.019–1.063). In vitro LDL susceptibility to oxidation was evaluated by following the kinetics of conjugated diene formation and by measuring the lag‐phase time in the presence of copper (Cu2+) ions. Results The percentages of glycated apoB (3.33 ± 2.54% vs. 1.24 ± 0.71%) and of LDL– (3.88  ± 1.49% vs. 2.34 ± 1.03%) in total LDL were significantly higher in diabetic patients (P 
ISSN:0742-3071
1464-5491
DOI:10.1046/j.1464-5491.1999.00136.x