Screening of folate status with use of dried blood spots on filter paper

Background: Dried blood spots (DBS) on filter paper have been a successful and economical matrix for neonatal screening. Objective: Our objective was to develop and evaluate an optimized method for DBS folate analysis and to assess DBS folate stability. Design: DBS were eluted from paper by sonicati...

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Bibliographic Details
Published in:The American journal of clinical nutrition 1999-09, Vol.70 (3), p.359-367
Main Authors: O'Broin, S.D, Gunter, E.W
Format: Article
Language:English
Subjects:
adults
assays
Biological and medical sciences
blood
Blood Stains
diagnostic techniques
Drug Stability
Erythrocytes - chemistry
evaluation
field experimentation
Filtration - instrumentation
folic acid
Folic Acid - blood
hematocrit
hemoglobin
Humans
Investigative techniques, diagnostic techniques (general aspects)
Linear Models
Mass Screening - methods
Medical sciences
Medical screening
Miscellaneous. Technology
Nutritional Status
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pregnancy
Reproducibility of Results
screening
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Summary:Background: Dried blood spots (DBS) on filter paper have been a successful and economical matrix for neonatal screening. Objective: Our objective was to develop and evaluate an optimized method for DBS folate analysis and to assess DBS folate stability. Design: DBS were eluted from paper by sonication in 5 g ascorbic acid/L containing 0.1% (by vol) Triton X-100 and hemoglobin folate values (HF; as pmol/g) were calculated from DBS eluate folate and hemoglobin concentrations. Results: Over 95% of DBS folate was eluted during a standardized sonication cycle and DBS folate assay reproducibility was acceptable both within (CV: < 8%) and between (CV: < 9%) runs. HF means (+/- 1 SD) from finger-stick DBS and conventional venous methods were 2513 +/- 1144 and 2607 +/- 1195 pmol/g, respectively, in blood samples taken concurrently from 80 donors, and they correlated well (r = 0.97, P < 0.001). HF values and erythrocyte folate measures may be interconverted by using the mean cell hemoglobin concentration. Conclusion: The DBS matrix has potential as an inexpensive and practical option for folate screening studies.
ISSN:0002-9165
1938-3207
DOI:10.1093/ajcn/70.3.359