Distribution and space-time relationship of proteoglycans in the extracellular matrix of the migratory pathway of primordial germ cells in mouse embryos

• Published in: Tissue & cell 1999-06, Vol.31 (3), p.291-300
• Main Authors: Soto-Suazo, M., Abrahamsohn, P.A., Pereda, J., Zorn, T.M.T.
• Format: Article
• Language: English
• Subjects: Animals; Cell Movement; Embryo, Mammalian - chemistry; Embryo, Mammalian - ultrastructure; Extracellular Matrix - chemistry; Extracellular Matrix - ultrastructure; Female; Germ Cells - chemistry; Germ Cells - physiology; Germ Cells - ultrastructure; Histocytochemistry; Male; Mice; Mice, Inbred BALB C; Microscopy, Electron; Primordial germ cells, cell migration, ruthenium hexammine trichloride, proteoglycans; Proteoglycans - analysis; Ruthenium Compounds; Time Factors
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1054/tice.1999.0041

In this paper we present an in situ ultrastructural cytochemical study on the distribution and spatial-temporal expression of proteoglycans (PGs) in the extracellular matrix of the migratory pathway of mouse primordial germ cells (PGCs) during the different phases of migration, by the use of the cationic dye ruthenium hexammine trichloride (RHT). Embryos of 9, 10, 11 and 12 days of development were used. The treatment with RHT revealed PGs as electron dense layers, granules, and filaments. Whereas granules prevailed in the extracellular spaces of the migratory route during the whole migratory process, the amount of filamentous structures increased during the migration phase of PGCs. At the end of the migratory process the surface of the PGCs lost its reaction by RHT. There were differences in the size of the granules of PGs at the initial migratory period (9-day-old embryos) as compared with the other days of gestation. There was a strong reaction for PGs in the extracellular spaces, expressed as a meshwork of granules interconnected by filaments, as well as reaction on the basement membranes during the peak of the PGCs migration in 10-day-old embryos. These results support the hypothesis that these molecules may have an important role in the migration of PGCs, although the precise mechanism involved in this process is not yet clear.