Cloning by somatic cell nuclear transfer

The birth of the first cloned mammals, produced by the introduction of somatic cell nuclei into enucleated oocytes, was an impressive and surprising development.(1) Although the ethical debate has been intense, the important scientific questions raised by this work have been inadequately discussed a...

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Bibliographic Details
Published in:BioEssays 1998-10, Vol.20 (10), p.847-851
Main Authors: Fulka Jr, Josef, First, Neal L., Loi, Pasqualino, Moor, Robert M.
Format: Article
Language:English
Subjects:
Animals
Animals, Domestic - embryology
Animals, Domestic - genetics
Calcium Signaling
Cattle
Cell Cycle
Chromatin - physiology
Cloning, Organism - methods
Cytoplasm - physiology
Embryo Transfer
Embryo, Mammalian - cytology
Embryo, Nonmammalian
Embryonic and Fetal Development
Forecasting
Mice - embryology
Mice - genetics
Nuclear Transfer Techniques
Ploidies
Rabbits
Sheep - embryology
Sheep - genetics
Species Specificity
Xenopus laevis - embryology
Xenopus laevis - genetics
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Summary:The birth of the first cloned mammals, produced by the introduction of somatic cell nuclei into enucleated oocytes, was an impressive and surprising development.(1) Although the ethical debate has been intense, the important scientific questions raised by this work have been inadequately discussed and are still unresolved. In this essay we address three questions about nuclear transplantation in the eggs of mice and domestic animals. First, why were the recent experiments on somatic cell cloning successful, when so many others have failed? Second, were these exceptional cases, or is somatic cloning now open to all? Third, what are the future possibilities for increasing the efficiency and wider applicability of the cloning process? BioEssays 20:847–851, 1998. © 1998 John Wiley & Sons, Inc.
ISSN:0265-9247
1521-1878
DOI:10.1002/(SICI)1521-1878(199810)20:10<847::AID-BIES10>3.0.CO;2-F