AOP-1 interacts with cardiac-specific protein kinase TNNI3K and down-regulates its kinase activity

In the present study, a yeast two-hybrid screening system was used to identify the interaction partners of cardiac troponin I-interacting kinase (TNNI3K) that might serve as regulators or targets, and thus in turn to gain some insights on the roles of TNNI3K. After screening the adult heart cDNA lib...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2007-11, Vol.72 (11), p.1199-1204
Main Authors: Feng, Yan, Liu, Dong-Qing, Wang, Zhen, Liu, Zhao, Cao, Hui-Qing, Wang, Lai-Yuan, Shi, Na, Meng, Xian-Min
Format: Article
Language:English
Subjects:
Antioxidants - chemistry
Antioxidants - metabolism
Baits
Base Sequence
Cell Line
DNA Primers - genetics
Down-Regulation
Humans
In Vitro Techniques
Kinases
MAP Kinase Kinase Kinases - antagonists & inhibitors
MAP Kinase Kinase Kinases - chemistry
MAP Kinase Kinase Kinases - genetics
MAP Kinase Kinase Kinases - metabolism
Myocardium - enzymology
Peroxiredoxin III
Peroxiredoxins - chemistry
Peroxiredoxins - genetics
Peroxiredoxins - metabolism
Protein Interaction Domains and Motifs
Protein Interaction Mapping
Proteins
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Transfection
Two-Hybrid System Techniques
Yeasts
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Summary:In the present study, a yeast two-hybrid screening system was used to identify the interaction partners of cardiac troponin I-interacting kinase (TNNI3K) that might serve as regulators or targets, and thus in turn to gain some insights on the roles of TNNI3K. After screening the adult heart cDNA library with a bait construct encoding the ANK motif of TNNI3K, antioxidant protein 1 (AOP-1) was isolated. The interaction between TNNI3K and AOP-1 was confirmed by the in vitro binding assay and coexpression experiments in vivo. The colocalization of TNNI3K and AOP-1 was clarified by confocal immunofluorescence. Moreover, coexpression of AOP-1 inhibited TNNI3K kinase activity in the in vitro kinase assay.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297907110053