Small subunit ribosomal RNA genes of tabanids and hippoboscids (Diptera: Brachycera): evolutionary relationships and comparison with other diptera

The small subunit ribosomal RNA (SSU rRNA) genes of hippoboscid (Ornithoica vicina Walker) and tabanid (Chrysops niger Macquart) Diptera were sequenced to determine their phylogenetic position within the order and to determine whether or not extensive hypervariable regions in this gene are widesprea...

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Bibliographic Details
Published in:Journal of medical entomology 1998-11, Vol.35 (6), p.1002-1006
Main Authors: Carreno, R.A, Barta, J.R
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chrysops
chyrsops niger
Diptera
Diptera - classification
Diptera - genetics
DNA, Ribosomal - genetics
Drosophila melanogaster - genetics
Fundamental and applied biological sciences. Psychology
genbank/af073888
genbank/af073889
Genes, Insect
genetic variation
Hippoboscidae
hypervariable region
Insecta
Invertebrates
molecular sequence data
nucleotide sequences
ornithoica vicina
Phylogeny
ribosomal DNA
ribosomal RNA
RNA, Ribosomal - genetics
Sequence Alignment
species differences
Systematics. Geographical distribution
Tabanidae
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Summary:The small subunit ribosomal RNA (SSU rRNA) genes of hippoboscid (Ornithoica vicina Walker) and tabanid (Chrysops niger Macquart) Diptera were sequenced to determine their phylogenetic position within the order and to determine whether or not extensive hypervariable regions in this gene are widespread in the Diptera. A parsimony analysis of an alignment containing 8 dipteran sequences produced a single most parsimonious tree that placed O. vicina as sister group to Drosophila melanogaster Meigen. The tabanid Chrysops niger was sister group to the asilomorphan taxa, and the sister group to the Brachycera was a Tipula sp. although this relationship was not supported by bootstrap analysis. The hippoboscid and tabanid sequences contain extensive hypervariable regions in the V2, V4, V6, and V7 regions as do other Diptera. When these regions of the alignment were excluded from the phylogenetic analysis, a single most parsimonious tree was found. This tree had an identical overall topology to the tree obtained from the total data set. The hypervariable regions in parts of the dipteran SSU rRNA genes were more extensive in the nematocerous dipteran sequences used in this study than in the other dipteran representatives; these hypervariable regions may be of more utility in inferring relationship among species and subspecies than at the suprageneric level.
ISSN:0022-2585
1938-2928
DOI:10.1093/jmedent/35.6.1002