Evolutionary insights inferred by molecular analysis of the ITS1-5.8S-ITS2 and IGS Avena sp. sequences

In an attempt to clarify phylogenetic and genome relationships among 35 diploid (A and C genomes), 13 tetraploid (AB and AC genomes) and 6 hexaploid (ACD genome) Avena taxa, 71 clones of the ITS1-5.8S-ITS2 fragment were sequenced, aligned and a network was constructed. In addition, the intergenic sp...

Full description

Saved in:
Bibliographic Details
Published in:Molecular phylogenetics and evolution 2008, Vol.46 (1), p.102-115
Main Authors: Nikoloudakis, Nikolaos, Skaracis, George, Katsiotis, Andreas
Format: Article
Language:English
Subjects:
Avena
Base Sequence
DNA, Plant - chemistry
DNA, Plant - genetics
Evolution
Evolution, Molecular
Genome, Plant - genetics
Intergenic spacer
Internal transcribed spacer
Molecular Sequence Data
Network
Phylogeny
Poaceae - classification
Poaceae - genetics
Polymorphism, Restriction Fragment Length
Polyploidy
rDNA
Sequence Analysis, DNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In an attempt to clarify phylogenetic and genome relationships among 35 diploid (A and C genomes), 13 tetraploid (AB and AC genomes) and 6 hexaploid (ACD genome) Avena taxa, 71 clones of the ITS1-5.8S-ITS2 fragment were sequenced, aligned and a network was constructed. In addition, the intergenic spacer (IGS) fragment was fingerprinted by means of a RFLP analysis using three different restriction enzymes. Both approaches led to comparable results. Clustering among the 54 Avena sp. entries was according to karyotype. Major genic divergence between the A and C genomes was revealed, while distinction among the A and B/D genomes was not possible. High affinity among the AB genome tetraploids and the A s genome diploid A. lusitanica was found, while AC genome tetraploids and ACD hexaploids were highly affiliated with the A l genome diploid A. longiglumis. The possible role of A. longiglumis in Avena sp. evolution is discussed.
ISSN:1055-7903
1095-9513
DOI:10.1016/j.ympev.2007.10.007