C. elegans AP-2 and Retromer Control Wnt Signaling by Regulating MIG-14/Wntless

While endocytosis can regulate morphogen distribution, its precise role in shaping these gradients is unclear. Even more enigmatic is the role of retromer, a complex that shuttles proteins between endosomes and the Golgi apparatus, in Wnt gradient formation. Here we report that DPY-23, the C. elegan...

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Published in:Developmental cell 2008-01, Vol.14 (1), p.132-139
Main Authors: Pan, Chun-Liang, Baum, Paul D., Gu, Mingyu, Jorgensen, Erik M., Clark, Scott G., Garriga, Gian
Format: Article
Language:English
Subjects:
Adaptor Protein Complex 2 - genetics
Adaptor Protein Complex 2 - physiology
Animals
Axons - physiology
Biological and medical sciences
Caenorhabditis elegans - physiology
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - physiology
Carrier Proteins - genetics
Carrier Proteins - physiology
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
CELLBIO
Endocytosis
Fundamental and applied biological sciences. Psychology
Homeostasis
Molecular and cellular biology
Mutation
Oligonucleotide Array Sequence Analysis
Phenotype
Signal transduction
SIGNALING
Transcription Factor AP-2 - physiology
Wnt Proteins - physiology
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Summary:While endocytosis can regulate morphogen distribution, its precise role in shaping these gradients is unclear. Even more enigmatic is the role of retromer, a complex that shuttles proteins between endosomes and the Golgi apparatus, in Wnt gradient formation. Here we report that DPY-23, the C. elegans μ subunit of the clathrin adaptor AP-2 that mediates the endocytosis of membrane proteins, regulates Wnt function. dpy-23 mutants display Wnt phenotypes, including defects in neuronal migration, neuronal polarity, and asymmetric cell division. DPY-23 acts in Wnt-expressing cells to promote these processes. MIG-14, the C. elegans homolog of the Wnt-secretion factor Wntless, also acts in these cells to control Wnt function. In dpy-23 mutants, MIG-14 accumulates at or near the plasma membrane. By contrast, MIG-14 accumulates in intracellular compartments in retromer mutants. Based on our observations, we propose that intracellular trafficking of MIG-14 by AP-2 and retromer plays an important role in Wnt secretion.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2007.12.001