Vasopressin-induced morphological changes in polarized rat hepatocyte multiplets: Dual calcium-dependent effects

Abstract Calcium-mobilizing hormones and neurotransmitters are known to affect cell morphology and function including cell differentiation or division. In this study, we examined vasopressin (AVP)-induced morphological changes in a polarized system of rat hepatocytes. Light and electron microscope o...

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Published in:Cell calcium (Edinburgh) 2008-01, Vol.43 (1), p.95-104
Main Authors: Serrière, Valérie, Tran, Dien, Stelly, Nicole, Claret, Michel, Alonso, Gérard, Tordjmann, Thierry, Guillon, Gilles
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - ultrastructure
Actins - analysis
Advanced Basic Science
Animals
Arginine Vasopressin - pharmacology
Bile Canaliculi - drug effects
Bile Canaliculi - ultrastructure
Calcium
Calcium - metabolism
Cell Polarity
Cells, Cultured
Female
Hepatocyte
Hepatocytes - drug effects
Hepatocytes - metabolism
Hepatocytes - ultrastructure
Microvilli - drug effects
Microvilli - ultrastructure
Polarity
Rats
Rats, Wistar
Vasopressin
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Summary:Abstract Calcium-mobilizing hormones and neurotransmitters are known to affect cell morphology and function including cell differentiation or division. In this study, we examined vasopressin (AVP)-induced morphological changes in a polarized system of rat hepatocytes. Light and electron microscope observations showed that AVP induced microvilli formation and a remodeling of the isolated hepatocyte F-actin submembrane cytoskeleton, these two events being correlated. We showed that these effects were rapid, reversible, observed at nanomolar AVP concentration and mediated by the V1a receptor. On polarized multicellular systems of hepatocytes, we observed a rapid reduction of the bile canaliculi lumen at the apical pole and micovilli formation at the basolateral domain with an enlarged F-actin cytoskeleton. Neither activation of protein kinase C nor A via phorbol ester or dibutyryl cAMP induced such rapid morphological changes, at variance with ionomycin, suggesting that AVP-induced intracellular calcium rise plays a crucial role in those effects. By using spectrofluorimetry and cytochemistry, we showed that calcium release from intracellular stores was involved in bile canaliculus contraction, while calcium entry from the extracellular space controlled microvilli formation. Taken together, AVP and calcium-mobilizing agonists differentially regulate physiological hepatocyte plasma membrane events at the basal and the apical domains via topographically specialized calcium-dependent mechanisms.
ISSN:0143-4160
1532-1991
DOI:10.1016/j.ceca.2007.04.008