Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma

The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. We studied the in vitro efflux of lipid...

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Bibliographic Details
Published in:Clinical biochemistry 2007-03, Vol.40 (5), p.305-309
Main Authors: Nikolić, Milan, Stanić, Dragana, Baričević, Ivona, Jones, David R., Nedić, Olgica, Niketić, Vesna
Format: Article
Language:English
Subjects:
Adult
Biological Transport
Cholesterol
Cholesterol - blood
Cholesterol - metabolism
Cholesterol, LDL - blood
Cholesterol, LDL - metabolism
Erythrocytes - metabolism
Fasting - blood
Haemoglobin
HDL cholesterol
Hemoglobins - metabolism
Humans
Lipoproteins
Male
Middle Aged
Phospholipids
Phospholipids - blood
Phospholipids - metabolism
Plasma - metabolism
Protein Binding
Red blood cells
Time Factors
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Summary:The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2006.11.005