Network activity-independent coordinated gene expression program for synapse assembly

Global biological datasets generated by genomics, transcriptomics, and proteomics provide new approaches to understanding the relationship between the genome and the synapse. Combined transcriptome analysis and multielectrode recordings of neuronal network activity were used in mouse embryonic prima...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-03, Vol.104 (11), p.4658-4663
Main Authors: Valor, Luis M, Charlesworth, Paul, Humphreys, Lawrence, Anderson, Chris N.G, Grant, Seth G.N
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Cell culture
Cells, Cultured
Developmental biology
Electrodes
Embryos
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Genes
Genome
Genomics
Hippocampus - embryology
Mice
Models, Biological
Neurons
Neurons - metabolism
Neuroscience
Oligonucleotide Array Sequence Analysis
Principal components analysis
Proteins
Proteomics - methods
RNA
Rodents
Synapses
Synapses - physiology
Time Factors
Transcription, Genetic
Transcriptomes
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Summary:Global biological datasets generated by genomics, transcriptomics, and proteomics provide new approaches to understanding the relationship between the genome and the synapse. Combined transcriptome analysis and multielectrode recordings of neuronal network activity were used in mouse embryonic primary neuronal cultures to examine synapse formation and activity-dependent gene regulation. Evidence for a coordinated gene expression program for assembly of synapses was observed in the expression of 642 genes encoding postsynaptic and plasticity proteins. This synaptogenesis gene expression program preceded protein expression of synapse markers and onset of spiking activity. Continued expression was followed by maturation of morphology and electrical neuronal networks, which was then followed by the expression of activity-dependent genes. Thus, two distinct sequentially active gene expression programs underlie the genomic programs of synapse function.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0609071104