Immunoisolating Pancreatic Islets by Encapsulation with Selective Withdrawal

This manuscript reports the application of the selective‐withdrawal coating technique to the microencapsulation of insulin‐producing pancreatic islets within thin poly(ethylene glycol) coatings. These polymer coatings permit the islets to respond to changes in glucose concentration by producing insu...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2007-04, Vol.3 (4), p.683-690
Main Authors: Wyman, Jason L., Kizilel, Seda, Skarbek, Ryan, Zhao, Xiangyang, Connors, Matthew, Dillmore, William S., Murphy, William L., Mrksich, Milan, Nagel, Sidney R., Garfinkel, Marc R.
Format: Article
Language:English
Subjects:
Animals
biomaterials
Capsules - chemistry
Coated Materials, Biocompatible - chemistry
coatings
diabetes
Diabetes Mellitus, Type 1 - therapy
Humans
Hydrogels - chemistry
Immune System
Islets of Langerhans - immunology
Islets of Langerhans - metabolism
Islets of Langerhans Transplantation - instrumentation
Islets of Langerhans Transplantation - methods
Lectins - chemistry
mesoporous materials
microcapsules
Microscopy, Confocal
Molecular Weight
Polyethylene Glycols - chemistry
Polymers - chemistry
Swine
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Summary:This manuscript reports the application of the selective‐withdrawal coating technique to the microencapsulation of insulin‐producing pancreatic islets within thin poly(ethylene glycol) coatings. These polymer coatings permit the islets to respond to changes in glucose concentration by producing insulin with a dose‐response profile that is substantially similar to that of unencapsulated islets. Furthermore, the hydrogel capsules exclude the large molecules of the immune system. These results suggest that the microencapsulation technique—which combines droplet formation from a flow of two immiscible fluids with polymerization chemistries—has the characteristics required for the transplantation of islets for the treatment of Type I diabetes. Isolation for transplantation: Transplantation of microencapsulated pancreatic islets has long been pursued as a therapy for Type I diabetes. Presented here is the application of a novel coating technique, capable of generating poly(ethylene glycol) coats of approximately 10 μm that protect the enclosed islets (see image) from large antibody‐sized molecules without inhibiting the diffusion of smaller insulin and glucose molecules.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200600231