investigation into the genetic background of coat colour dilution in a Charolais x German Holstein F₂ resource population

The molecular background of many loci affecting coat colour inheritance in cattle is still incompletely characterized, although it is known that coat colour results from the joint effects of several loci, e.g. agouti, extension and dilution. Dilution alleles are responsible for a dilution effect on...

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Bibliographic Details
Published in:Animal genetics 2007-04, Vol.38 (2), p.109-113
Main Authors: Kühn, Ch, Weikard, R
Format: Article
Language:English
Subjects:
Animals
Base Sequence
cattle
Cattle - genetics
Cattle - physiology
Chromosome Mapping
coat colour
Crosses, Genetic
dilution
DNA Primers
Genetic Linkage
gp100 Melanoma Antigen
Hair - physiology
Membrane Glycoproteins - genetics
Microsatellite Repeats - genetics
Molecular Sequence Data
Pigmentation - genetics
Pigmentation - physiology
Point Mutation - genetics
Sequence Analysis, DNA
SILV
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Summary:The molecular background of many loci affecting coat colour inheritance in cattle is still incompletely characterized, although it is known that coat colour results from the joint effects of several loci, e.g. agouti, extension and dilution. Dilution alleles are responsible for a dilution effect on the original coat colour of an individual, which is determined by the agouti and extension loci. Different loci affecting dilution of pigment are suggested in Charolais (Dc) and Simmental (Ds). To enable chromosomal mapping of the Dc mutation, 133 animals from an F₂ full-sib resource population generated from a cross of Charolais and German Holstein were scored for the coat colour dilution phenotype. Linkage analysis covering all autosomes revealed a significant linkage of the dilution phenotype with microsatellite markers on bovine chromosome 5. No recombination was observed between marker ETH10 and the Dc locus. Positional and functional information identified the bovine silver homolog (SILV) gene as a candidate for the Dc mutation. Results from comparative sequencing of the SILV gene in individuals with different dilution coat colour phenotypes confirmed the presence of a c.64G>A non-synonymous mutation, which had previously been identified in the Charolais breed. The alleles at this locus were associated with coat colour dilution in this study. However, further investigation of colour inheritance within the F₂ resource population indicated that a single diallelic mutation in the SILV gene cannot explain the total observed variation of coat colour dilution.
ISSN:0268-9146
1365-2052
DOI:10.1111/j.1365-2052.2007.01569.x