DrRRA: a novel response regulator essential for the extreme radioresistance of Deinococcus radiodurans

• Published in: Molecular microbiology 2008-03, Vol.67 (6), p.1211-1222
• Main Authors: Wang, Liangyan, Xu, Guangzhi, Chen, Huan, Zhao, Ye, Xu, Nan, Tian, Bing, Hua, Yuejin
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biochemistry; Biological and medical sciences; Blotting, Western; Catalase - genetics; Catalase - metabolism; Deinococcus - genetics; Deinococcus - metabolism; Deinococcus - radiation effects; Electrophoresis, Gel, Pulsed-Field; Electrophoresis, Polyacrylamide Gel; Eukaryotes; Fundamental and applied biological sciences. Psychology; Gamma Rays; Gene Expression Regulation, Bacterial - radiation effects; Genome, Bacterial; Kinases; Microbiology; Miscellaneous; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Reactive Oxygen Species - metabolism; Rec A Recombinases - genetics; Rec A Recombinases - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Signal transduction; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/j.1365-2958.2008.06113.x

Two-component systems are predominant signal transduction pathways in prokaryotes, and also exist in many archaea as well as some eukaryotes. A typical TCS consists of a histidine kinase and a cognate response regulator. In this study, a novel gene encoding a response regulator (we designate it drRRA) is identified to be essential for the extreme radioresistance of Deinococcus radiodurans. DrRRA null mutant (we designate it MR) is sensitive to gamma-radiation compared with the wild-type strain. Transcriptional assays show that numerous genes are changed in their transcriptional levels in MR at exponential growth phase under normal or gamma-radiation condition. Most of them are related to stress response and DNA repair. Antioxidant activity assays exhibit that both superoxide dismutases and catalases are decreased in the mutant, whereas Western blotting assays show that RecA and PprA are also reduced in MR, verifying the microarray and quantitative real-time PCR data. Furthermore, pulsed-field gel electrophoresis assay demonstrates that deletion of drRRA results in the delay of genome restitution. These data support the hypothesis that DrRRA contributes to the extreme radioresistance of D. radiodurans through its regulatory role in multiple pathways such as antioxidation and DNA repair pathways.