Electrochemical Quartz Crystal Impedance and Fluorescence Quenching Studies on the Binding of Carbon Nanotubes (CNTs)-Adsorbed and Solution Rutin with Hemoglobin

• Published in: Biotechnology progress 2007-03, Vol.23 (2), p.473-479
• Main Authors: Su, Yuhua, Xie, Qingji, Yang, Qin, Tu, Xinman, Cao, Zhijun, Jia, Xue'en, Su, Zhaohong, Zhang, Youyu, Meng, Wenhua, Yao, Shouzhuo
• Format: Article
• Language: English
• Subjects: Adsorption; Binding Sites; Biological and medical sciences; Biotechnology; Computer Simulation; Electric Impedance; Electrochemistry - methods; Fundamental and applied biological sciences. Psychology; Hemoglobins - chemistry; Microscopy, Fluorescence - methods; Models, Chemical; Nanotubes, Carbon - chemistry; Protein Binding; Quartz; Rutin - chemistry; Solubility; Solutions
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp0603356

Electrochemical quartz crystal impedance (QCI) technique was utilized to monitor in situ the adsorption of rutin (RT) onto a carbon nanotubes (CNTs)‐modified gold electrode and to study the binding process of solution hemoglobin (Hb) to RT immobilized on the electrode. Time courses of the QCI parameters including crystal resonant frequency were simultaneously obtained during the RT adsorption and Hb‐RT binding. In contrast to the negligible RT adsorption at a bare gold electrode, the modification by CNTs notably enhanced the amount of adsorption, and almost all of the adsorbed RT molecules were found to be electroactive. On the basis of the frequency response from the binding of adsorbed RT to solution Hb and the diminished electroactivity of adsorbed RT after the formation of the electrochemically inactive RT‐Hb adduct, the average binding molar ratio of adsorbed RT to Hb was estimated to be 23.9:1, and the association constant (Ka) for the binding was estimated to be 2.87 × 106 (frequency) and 3.92 × 106 (charge) L mol−1, respectively. Comparable results were obtained from fluorescence quenching measurements in mixed solutions containing RT of fixed concentration and Hb of varying concentrations, demonstrating that the interfacial RT here behaved equivalently in the RT‐Hb binding activity compared to that in solution. This work may have presented a new and general protocol involving CNTs to study many other electroactive natural antioxidants or drugs that are at the interface or in solution, their binding with proteins or other biomolecules, and changes of their antioxidant activity after the binding.