PTPRK negatively regulates transcriptional activity of wild type and mutated oncogenic β-catenin and affects membrane distribution of β-catenin/E-cadherin complexes in cancer cells

• Published in: Cellular signalling 2008-05, Vol.20 (5), p.872-883
• Main Authors: Novellino, Luisa, De Filippo, Annamaria, Deho, Paola, Perrone, Federica, Pilotti, Silvana, Parmiani, Giorgio, Castelli, Chiara
• Format: Article
• Language: English
• Subjects: beta Catenin - chemistry; beta Catenin - genetics; beta Catenin - metabolism; Cadherins - chemistry; Cadherins - metabolism; Cell Line, Tumor; E-cadherin; Genes, Tumor Suppressor; Humans; Melanoma; Melanoma - genetics; Melanoma - metabolism; Melanoma - pathology; Melanoma - secondary; Models, Biological; Multiprotein Complexes; Mutation; PTPRK; Receptor-Like Protein Tyrosine Phosphatases, Class 2 - antagonists & inhibitors; Receptor-Like Protein Tyrosine Phosphatases, Class 2 - genetics; Receptor-Like Protein Tyrosine Phosphatases, Class 2 - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; RNA, Small Interfering - genetics; Signal Transduction; Transcription, Genetic; Tumor suppressor; β-catenin
• ISSN: 0898-6568; 1873-3913
• DOI: 10.1016/j.cellsig.2007.12.024

Previous reports showed that receptor-type protein-tyrosine phosphatase PTPRK co-localizes with β-catenin at adherens junctions, and in vitro experiments suggested that β-catenin could be substrate of PTPRK-mediated phosphatase activity. β-catenin is a molecule endowed with a dual function being involved both in cell adhesion and in Wnt signaling pathway. Here we provide evidence for the role of PTPRK in negatively regulating the β-catenin transcriptional activity by modulating its intracellular and membrane distribution. Expression of PTPRK protein in HEK293 cells and in PTPRK-null melanoma cell lines, one of which harbors a mutated oncogenic β-catenin, impairs nuclear accumulation of wild type and oncogenic forms of β-catenin, limits cytosolic levels of tyrosine-phosphorylated β-catenin, and leads to re-localization of E-cadherin/β-catenin complexes in ordered membrane phase along cell–cell contacts. This re-modulation of β-catenin cellular distribution results in the inhibition of cyclin D1 and c-myc protein expression, whose genes are targets of β-catenin. Tumor cells upon re-expression of PTPRK have a reduced proliferative and migration capacity. Moreover we show that PTPRK is also active in negatively regulating the transactivating function of β-catenin in normal melanocytes as confirmed by experiments with silenced PTPRK by specific siRNA. Our data show that PTPRK influences transactivating activity of β-catenin in non-tumoral and neoplastic cells by regulating the balance between signaling and adhesive β-catenin, thus providing biochemical basis for the hypothesis of PTPRK as a tumor suppressor gene.