Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vitro

• Published in: Biology of the cell 2008-04, Vol.100 (4), p.231-241
• Main Authors: Vieira, Natássia M., Brandalise, Vanessa, Zucconi, Eder, Jazedje, Tatiana, Secco, Mariane, Nunes, Viviane A., Strauss, Bryan E., Vainzof, Mariz, Zatz, Mayana
• Format: Article
• Language: English
• Subjects: adipose; Adipose Tissue - cytology; Blotting, Western; Cell Culture Techniques; Cell Differentiation; Cell Separation; Coculture Techniques; DMD therapy; Duchenne muscular dystrophy (DMD); Dystrophin - metabolism; Flow Cytometry; Gene Expression; Humans; in vitro study; Multipotent Stem Cells - cytology; Muscle Cells - cytology; Muscle Development; Muscle Fibers, Skeletal - cytology; Muscular Dystrophy, Duchenne - metabolism; Myoblasts - cytology; Reverse Transcriptase Polymerase Chain Reaction; skeletal-muscle cell; stem cell; Stem Cells - cytology; Transduction, Genetic
• ISSN: 0248-4900; 1768-322X
• DOI: 10.1042/BC20070102

Background information. DMD (Duchenne muscular dystrophy) is a devastating X‐linked disorder characterized by progressive muscle degeneration and weakness. The use of cell therapy for the repair of defective muscle is being pursued as a possible treatment for DMD. Mesenchymal stem cells have the potential to differentiate and display a myogenic phenotype in vitro. Since liposuctioned human fat is available in large quantities, it may be an ideal source of stem cells for therapeutic applications. ASCs (adipose‐derived stem cells) are able to restore dystrophin expression in the muscles of mdx (X‐linked muscular dystrophy) mice. However, the outcome when these cells interact with human dystrophic muscle is still unknown. Results. We show here that ASCs participate in myotube formation when cultured together with differentiating human DMD myoblasts, resulting in the restoration of dystrophin expression. Similarly, dystrophin was induced when ASCs were co‐cultivated with DMD myotubes. Experiments with GFP (green fluorescent protein)‐positive ASCs and DAPI (4′,6‐diamidino‐2‐phenylindole)‐stained DMD myoblasts indicated that ASCs participate in human myogenesis through cellular fusion. Conclusions. These results show that ASCs have the potential to interact with dystrophic muscle cells, restoring dystrophin expression of DMD cells in vitro. The possibility of using adipose tissue as a source of stem cell therapies for muscular diseases is extremely exciting.