Gene expression profiling of the pH response in Shigella flexneri 2a

The pH response of Shigella flexneri 2a 301 was identified by gene expression profiling. Gene expression profiles of cells grown in pH 4.5 or 8.6 were compared with the profiles of cells grown at pH 7.0. Differential expression was observed for 307 genes: 97 were acid up-regulated, 102 were acid dow...

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Bibliographic Details
Published in:FEMS microbiology letters 2007-05, Vol.270 (1), p.12-20
Main Authors: Cheng, Fan, Wang, Jing, Peng, Junping, Yang, Jian, Fu, Hua, Zhang, Xiaobing, Xue, Ying, Li, Weijun, Chu, Yonglie, Jin, Qi
Format: Article
Language:English
Subjects:
Acid resistance
Acids
Acids - pharmacology
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Energy metabolism
Fundamental and applied biological sciences. Psychology
Gene expression
gene expression profiling
Gene Expression Profiling - methods
Gene Expression Regulation, Bacterial - drug effects
Genes
Genetics
Hydrogen-Ion Concentration
Microbiology
Oligonucleotide Array Sequence Analysis
pH effects
pH response
Shigella flexneri
Shigella flexneri - drug effects
Shigella flexneri - genetics
Virulence
Virulence - genetics
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Summary:The pH response of Shigella flexneri 2a 301 was identified by gene expression profiling. Gene expression profiles of cells grown in pH 4.5 or 8.6 were compared with the profiles of cells grown at pH 7.0. Differential expression was observed for 307 genes: 97 were acid up-regulated, 102 were acid down-regulated, 91 were base up-regulated, and 86 were base down-regulated. Twenty-seven genes were found to be both acid and base up-regulated, and 29 genes were both acid and base down-regulated. This study showed that (1) the most pH-dependent genes regulate energy metabolism; (2) the RpoS-dependent acid-resistance system is induced, while the glutamate-dependent acid resistance system is not; (3) high pH up-regulates some virulence genes, while low pH down-regulates them, consistent with Shigella infection of the low gut; and (4) several cross-stress response genes are induced by pH changes. These results also illustrate that many unknown genes are significantly regulated under acid or basic conditions, providing researchers with important information to characterize their function.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2007.00647.x