Decreased expression of phospholipase C-beta 1 protein in endoplasmic reticulum stress-loaded neurons

• Published in: Biological & pharmaceutical bulletin 2008-04, Vol.31 (4), p.719-719
• Main Authors: Yasuda, Eri, Nagasawa, Kazuki, Nishida, Kentaro, Fujimoto, Sadaki
• Format: Article
• Language: English
• Subjects: Animals; Blotting, Western; Calcimycin - pharmacology; Calcium - metabolism; Caspase 12 - drug effects; Caspase 12 - metabolism; Caspase 3 - metabolism; Cell Survival - drug effects; Cells, Cultured; Endoplasmic Reticulum - enzymology; Enzyme Inhibitors - pharmacology; Neurons - enzymology; Phospholipase C beta - biosynthesis; Phospholipase C gamma - biosynthesis; Rats; Stress, Physiological - enzymology; Tetrazolium Salts; Thapsigargin - pharmacology; Thiazoles; Tunicamycin - pharmacology
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.31.719

The endoplasmic reticulum (ER) plays a critical role in the maintenance of intracellular homeostasis and its dysfunction is thought to lead to neuronal death, which results in neurodegenerative disorders. Since phospholipase C (PLC) isozymes are involved in maintenance of the intracellular Ca2+ concentration by regulating Ca2+ release from the ER, their expression might be affected by ER stress. Of these isozymes, PLC-beta 1 and -gamma 1, in particular, are known to protect cells from oxidative stress and thus alteration of their expression profile under ER stress-loaded conditions is interesting. Using primary cultured rat cortical neurons, we here examined whether expression of PLC-beta 1 and -gamma 1 was altered in ER stress-loaded neurons induced by tunicamycin (Tm). In ER stress-loaded neurons treated with Tm in the range of 0.03-3 microg/ml for 20 h, the viability of the neurons was decreased dose-dependently, the decrease being significant with 0.3 or more microg/ml, and expression of the representative ER stress markers, GRP78/BiP, and cleaved caspase-3 and -12, was increased after 24 h postincubation, confirming the induction of ER stress in the neurons. In the ER stress-loaded neurons obtained on Tm treatment, the expression level of PLC-beta 1 decreased dose-dependently. On the other hand, there was no difference in the PLC-gamma 1 protein expression level between control and ER stress-loaded neurons. Overall, we demonstrated that ER stress decreases the expression of PLC-beta 1, but not -gamma 1, in neurons.