Insulin resistance following thermal injury: An animal study

• Published in: Burns 2007-06, Vol.33 (4), p.480-483
• Main Authors: Xin-Long, Chen, Zhao-Fan, Xia, Dao-Feng, Ben, Jian-Guang, Tian, Duo, Wei
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blood Glucose - metabolism; Burn; Burns; Burns - blood; Critical Care; Euglycemic–hyperinsulinemic glucose clamps; Glucose Clamp Technique; Glucose Tolerance Test; Glucose tolerance tests; Hypoglycemic Agents - administration & dosage; Insulin - administration & dosage; Insulin - metabolism; Insulin resistance; Insulin Resistance - physiology; Insulin sensitivity index; Medical sciences; Rats; Rats, Sprague-Dawley; Traumas. Diseases due to physical agents
• ISSN: 0305-4179; 1879-1409
• DOI: 10.1016/j.burns.2006.08.017

Abstract The aim of this study was to investigate the changes of glucose tolerance, insulin sensitivity, and euglycemic–hyperinsulinemic glucose clamps following a 30% TBSA full thickness third degree burn in rats. Sprague–Dawley rats weighing 160–170 g received 30% TBSA full thickness third degree burn by immersing the back of trunk for 12 s in a boiling water bath under anesthesia. Weight- and time-matched sham burn group (control) was treated in the same manner as the trauma group, except that they were immersed in a room-temperature water bath. After 12 h overnight fasting, plasma insulin concentration was determined by ELISA using rat-insulin enzyme immunoassay kit (SPI-BIO) and blood glucose was assayed by glucose analyzer at 3 days after burn. Insulin sensitivity index was calculated by using slightly modified formula. The rat was injected with 5% glucose (2 g/kg body weight, intraperitoneally) to observe the change of glucose tolerance at 3 days after burn. Euglycemic–hyperinsulinemic glucose clamps were performed at 4 days after burn. Insulin sensitivity index of burn group was significantly reduced compared with control group at 3 days after burn (0.58 ± 0.23 versus 1.23 ± 0.16, P < 0.01). The significant difference of glucose tolerance was observed between the two groups and the glucose infused rate measured in burned rats was significantly decreased compared with that in control at 4 days after injury (7.23 ± 1.35 versus 12.31 ± 0.54, P < 0.01). Conclusion : Burn causes the significant change of glucose metabolism and results in insulin resistance in rats.