Covalent Grafting of Fibronectin onto Plasma-Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity

Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(p‐maleimidophenyl)butyrate (su...

Full description

Saved in:
Bibliographic Details
Published in:Macromolecular bioscience 2007-05, Vol.7 (5), p.738-745
Main Authors: Vallières, Karine, Petitclerc, Éric, Laroche, Gaétan
Format: Article
Language:English
Subjects:
Ammonia
Animals
Aorta - cytology
bioactivity
Biocompatible Materials
Cattle
Cell Adhesion
crosslinking
Endothelial Cells - cytology
Enzyme-Linked Immunosorbent Assay
fibronectin
Fibronectins - chemistry
Glutarates - chemistry
Plasma
polytetrafluoroethylene (PTFE)
Polytetrafluoroethylene - chemistry
Protein Conformation
Succinimides - chemistry
surface modification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c4787-9ce2364b3d74c6a0f5fa1eb50745c2d092e590b0b2d885941780066c4c625bfb3
cites cdi_FETCH-LOGICAL-c4787-9ce2364b3d74c6a0f5fa1eb50745c2d092e590b0b2d885941780066c4c625bfb3
container_end_page 745
container_issue 5
container_start_page 738
container_title Macromolecular bioscience
container_volume 7
creator Vallières, Karine
Petitclerc, Éric
Laroche, Gaétan
description Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(p‐maleimidophenyl)butyrate (sulfo‐SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE‐GA‐FN than on PTFE‐SMPB‐FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.
doi_str_mv 10.1002/mabi.200600267
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70510852</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20867836</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4787-9ce2364b3d74c6a0f5fa1eb50745c2d092e590b0b2d885941780066c4c625bfb3</originalsourceid><addsrcrecordid>eNqFkcFv0zAUxi0EYmNw5Yh84pZiO7GdcGvLGioNmLQijpbtOMUjsTfbGfTMP46rVgVOO9nvvd_3Se99ALzGaIYRIu9GqeyMIMRywfgTcI4ZZgXFDX16-tf8DLyI8RYhzOuGPAdnmFeUNxU9B7-X_kEOxiXYBtkn67bQ93BlVfDO6FxD75KH14OMoyw2wchkOni9WV2-h2vXD5Nx2uwl6buBS-9up61M1jt4k0JGt7us_89uYf3gt1bLAc5z48Gm3UvwrJdDNK-O7wX4urrcLD8WV1_a9XJ-VeiK17xotCElq1TZ8UoziXraS2wURXkZTTrUEEMbpJAiXV3TpsrL5rswnWFCVa_KC_D24HsX_P1kYhKjjdoMg3TGT1FwRDGqKXkUJKhmvC5ZBmcHUAcfYzC9uAt2lGEnMBL7fMQ-H3HKJwveHJ0nNZruL34MJAPNAfhpB7N7xE58mi_W_5oXB62Nyfw6aWX4IfKUU_Htcyvali3oB74SN-UfS3WsQg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20867836</pqid></control><display><type>article</type><title>Covalent Grafting of Fibronectin onto Plasma-Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity</title><source>Wiley</source><creator>Vallières, Karine ; Petitclerc, Éric ; Laroche, Gaétan</creator><creatorcontrib>Vallières, Karine ; Petitclerc, Éric ; Laroche, Gaétan</creatorcontrib><description>Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(p‐maleimidophenyl)butyrate (sulfo‐SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE‐GA‐FN than on PTFE‐SMPB‐FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.200600267</identifier><identifier>PMID: 17457945</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Ammonia ; Animals ; Aorta - cytology ; bioactivity ; Biocompatible Materials ; Cattle ; Cell Adhesion ; crosslinking ; Endothelial Cells - cytology ; Enzyme-Linked Immunosorbent Assay ; fibronectin ; Fibronectins - chemistry ; Glutarates - chemistry ; Plasma ; polytetrafluoroethylene (PTFE) ; Polytetrafluoroethylene - chemistry ; Protein Conformation ; Succinimides - chemistry ; surface modification</subject><ispartof>Macromolecular bioscience, 2007-05, Vol.7 (5), p.738-745</ispartof><rights>Copyright © 2007 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4787-9ce2364b3d74c6a0f5fa1eb50745c2d092e590b0b2d885941780066c4c625bfb3</citedby><cites>FETCH-LOGICAL-c4787-9ce2364b3d74c6a0f5fa1eb50745c2d092e590b0b2d885941780066c4c625bfb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17457945$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vallières, Karine</creatorcontrib><creatorcontrib>Petitclerc, Éric</creatorcontrib><creatorcontrib>Laroche, Gaétan</creatorcontrib><title>Covalent Grafting of Fibronectin onto Plasma-Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(p‐maleimidophenyl)butyrate (sulfo‐SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE‐GA‐FN than on PTFE‐SMPB‐FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.</description><subject>Ammonia</subject><subject>Animals</subject><subject>Aorta - cytology</subject><subject>bioactivity</subject><subject>Biocompatible Materials</subject><subject>Cattle</subject><subject>Cell Adhesion</subject><subject>crosslinking</subject><subject>Endothelial Cells - cytology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>fibronectin</subject><subject>Fibronectins - chemistry</subject><subject>Glutarates - chemistry</subject><subject>Plasma</subject><subject>polytetrafluoroethylene (PTFE)</subject><subject>Polytetrafluoroethylene - chemistry</subject><subject>Protein Conformation</subject><subject>Succinimides - chemistry</subject><subject>surface modification</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkcFv0zAUxi0EYmNw5Yh84pZiO7GdcGvLGioNmLQijpbtOMUjsTfbGfTMP46rVgVOO9nvvd_3Se99ALzGaIYRIu9GqeyMIMRywfgTcI4ZZgXFDX16-tf8DLyI8RYhzOuGPAdnmFeUNxU9B7-X_kEOxiXYBtkn67bQ93BlVfDO6FxD75KH14OMoyw2wchkOni9WV2-h2vXD5Nx2uwl6buBS-9up61M1jt4k0JGt7us_89uYf3gt1bLAc5z48Gm3UvwrJdDNK-O7wX4urrcLD8WV1_a9XJ-VeiK17xotCElq1TZ8UoziXraS2wURXkZTTrUEEMbpJAiXV3TpsrL5rswnWFCVa_KC_D24HsX_P1kYhKjjdoMg3TGT1FwRDGqKXkUJKhmvC5ZBmcHUAcfYzC9uAt2lGEnMBL7fMQ-H3HKJwveHJ0nNZruL34MJAPNAfhpB7N7xE58mi_W_5oXB62Nyfw6aWX4IfKUU_Htcyvali3oB74SN-UfS3WsQg</recordid><startdate>20070510</startdate><enddate>20070510</enddate><creator>Vallières, Karine</creator><creator>Petitclerc, Éric</creator><creator>Laroche, Gaétan</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070510</creationdate><title>Covalent Grafting of Fibronectin onto Plasma-Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity</title><author>Vallières, Karine ; Petitclerc, Éric ; Laroche, Gaétan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4787-9ce2364b3d74c6a0f5fa1eb50745c2d092e590b0b2d885941780066c4c625bfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Ammonia</topic><topic>Animals</topic><topic>Aorta - cytology</topic><topic>bioactivity</topic><topic>Biocompatible Materials</topic><topic>Cattle</topic><topic>Cell Adhesion</topic><topic>crosslinking</topic><topic>Endothelial Cells - cytology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>fibronectin</topic><topic>Fibronectins - chemistry</topic><topic>Glutarates - chemistry</topic><topic>Plasma</topic><topic>polytetrafluoroethylene (PTFE)</topic><topic>Polytetrafluoroethylene - chemistry</topic><topic>Protein Conformation</topic><topic>Succinimides - chemistry</topic><topic>surface modification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vallières, Karine</creatorcontrib><creatorcontrib>Petitclerc, Éric</creatorcontrib><creatorcontrib>Laroche, Gaétan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vallières, Karine</au><au>Petitclerc, Éric</au><au>Laroche, Gaétan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Covalent Grafting of Fibronectin onto Plasma-Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2007-05-10</date><risdate>2007</risdate><volume>7</volume><issue>5</issue><spage>738</spage><epage>745</epage><pages>738-745</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(p‐maleimidophenyl)butyrate (sulfo‐SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE‐GA‐FN than on PTFE‐SMPB‐FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>17457945</pmid><doi>10.1002/mabi.200600267</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1616-5187
ispartof Macromolecular bioscience, 2007-05, Vol.7 (5), p.738-745
issn 1616-5187
1616-5195
language eng
recordid cdi_proquest_miscellaneous_70510852
source Wiley
subjects Ammonia
Animals
Aorta - cytology
bioactivity
Biocompatible Materials
Cattle
Cell Adhesion
crosslinking
Endothelial Cells - cytology
Enzyme-Linked Immunosorbent Assay
fibronectin
Fibronectins - chemistry
Glutarates - chemistry
Plasma
polytetrafluoroethylene (PTFE)
Polytetrafluoroethylene - chemistry
Protein Conformation
Succinimides - chemistry
surface modification
title Covalent Grafting of Fibronectin onto Plasma-Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T22%3A01%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Covalent%20Grafting%20of%20Fibronectin%20onto%20Plasma-Treated%20PTFE:%20Influence%20of%20the%20Conjugation%20Strategy%20on%20Fibronectin%20Biological%20Activity&rft.jtitle=Macromolecular%20bioscience&rft.au=Valli%C3%A8res,%20Karine&rft.date=2007-05-10&rft.volume=7&rft.issue=5&rft.spage=738&rft.epage=745&rft.pages=738-745&rft.issn=1616-5187&rft.eissn=1616-5195&rft_id=info:doi/10.1002/mabi.200600267&rft_dat=%3Cproquest_cross%3E20867836%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4787-9ce2364b3d74c6a0f5fa1eb50745c2d092e590b0b2d885941780066c4c625bfb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20867836&rft_id=info:pmid/17457945&rfr_iscdi=true