Continuously Microscopically Observed and Process-Controlled Cell Culture Within the SlideReactor: Proof of a New Concept for Cell Characterization

Certain cell types, especially primary human cells, favor a well-defined culture environment offering continuous supply of nutrients and oxygen and waste product removal. Several bioreactors based on special matrices or hollow fibers have been developed that provide such conditions. However, charact...

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Bibliographic Details
Published in:Tissue engineering 2007-01, Vol.13 (1), p.187-196
Main Authors: Schwartlander, Ruth, Schmid, Jochen, Brandenburg, Börries, Katenz, Ekaterina, Vondran, Florian Wolfgang Rudolf, Pless, Gesine, Cheng, Xiangdong, Pascher, Andreas, Neuhaus, Peter, Sauer, Igor Maximilian
Format: Article
Language:English
Subjects:
Bioreactors
Cell Adhesion - physiology
Cell Culture Techniques - instrumentation
Cell Line
Cell Line, Tumor
Cells, Cultured
Hepatocytes - cytology
Hepatocytes - physiology
Hepatocytes - ultrastructure
Humans
Hypothermia, Induced
Microscopy, Confocal
Microscopy, Fluorescence
Microscopy, Video - instrumentation
Microscopy, Video - methods
Skin - cytology
Skin - ultrastructure
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Summary:Certain cell types, especially primary human cells, favor a well-defined culture environment offering continuous supply of nutrients and oxygen and waste product removal. Several bioreactors based on special matrices or hollow fibers have been developed that provide such conditions. However, characterization of matrix re-organization or growth of tissue within these systems is possible only after culture termination. Evaluation of the influence of certain medium additives or culture conditions (e.g., temperature, oxygenation) on cell viability, expansion, and differentiation within these systems remains a challenging task. The SlideReactor , a miniaturized hollow fiber-based bioreactor, was developed to enable the observation of cells during culture. An operation concept offering predefined conditions for various cell types has been designed. For proof of concept, primary human cells (hepatocytes, fibroblasts, keratinocytes) and cell lines (HepG2, HuH7, C3A, WiDr, SkHep1) were cultured and observed. A series of experiments (n = 40) showed the feasibility of the set-up; determination of process parameters and continuous observation is possible. The SlideReactor may serve as a simple and cost-efficient tool for cell characterization and optimization of cell-culture conditions.
ISSN:1076-3279
1557-8690
DOI:10.1089/ten.2006.0071