Elastic plasma protein film blended with platelet releasate accelerates healing of diabetic mouse skin wounds

• Published in: Vox sanguinis 2007-07, Vol.93 (1), p.49-56
• Main Authors: Tanaka, R., Ichioka, S., Sekiya, N., Ohura, N., Uchino, S., Ojima, A., Itoh, Y., Ishihara, O., Nakatsuka, T., Ikebuchi, K.
• Format: Article
• Language: English
• Subjects: angiogenesis; Animals; autologous biological dressing; Biological Dressings; Blood Platelets - chemistry; Blood Proteins - therapeutic use; Cell Extracts - chemistry; Cell Extracts - therapeutic use; Diabetes Complications - pathology; Diabetes Complications - therapy; Diabetes Mellitus, Type 2 - pathology; Diabetes Mellitus, Type 2 - therapy; Disease Models, Animal; Elasticity; Humans; Membranes, Artificial; Mice; platelet- and plasma-derived factors; Skin - injuries; Skin - pathology; Skin Diseases - pathology; Skin Diseases - therapy; Wound Healing; Wounds and Injuries - pathology; Wounds and Injuries - therapy
• ISSN: 0042-9007; 1423-0410
• DOI: 10.1111/j.1423-0410.2007.00913.x

Background and Objectives  The growth factors derived from platelets and plasma proteins mediate the wound‐healing process that is characterized by the sequential migration and differentiation of several cell populations that give rise to angiogenesis, collagen synthesis, wound contraction, and re‐epithelialization. To evaluate the efficacy of the blood‐derived factors in wound healing, we examined a novel wound dressing consisting of concentrated human plasma proteins and platelet releasate (CPPP). Materials and Methods  To generate CPPP, plasma proteins and platelets in the peripheral blood (n = 5) were concentrated with the cold ethanol precipitation method. The thrombin obtained from the same blood unit and calcium chloride (CaCl2) were mixed to a concentrate. The CPPP has enough strength to dress cutaneous wounds and contains large amounts of cytokines and fibronectin. We applied the CPPP to excisional skin wounds in genetically healing‐impaired model mice (n= 5) and the wounds were evaluated 10 days after the operation. Results  The area of CPPP‐treated wounds decreased significantly compared with that of the control wounds (65% vs. 94% of the original size, respectively, P= 0·032). The immunostained section revealed a striking effect of CPPP on vascularization compared with the control wounds (13·2 vs. 2·7 vessels per mm2 as mean vascular density observed in the sections, respectively, P= 0·013). Conclusions  Our results suggest that CPPP is a promising biologically active dressing for full‐thickness skin wounds. CPPP can be an entirely autologous biological dressing, suggesting that it is free from the risk of transmission of pathogens through blood products.