Selection of oral microbial adhesion antagonists using biotinylated Streptococcus sanguis and a human mixed oral microflora

A microtitre assay has been developed using hydroxyapatite-coated wells and Streptococcus sanguis NCTC 10904 at 10 7 cells per ml. A number of models representing toothpaste and mouthwash usage were adopted to detect the anti-adherent efficacy of a polyvinylmethylether maleic acid copolymer (PVM/MA)...

Full description

Saved in:
Bibliographic Details
Published in:Archives of oral biology 2001-02, Vol.46 (2), p.129-138
Main Authors: Guan, Y.H., de Graaf, T., Lath, D.L., Humphreys, S.M., Marlow, I., Brook, A.H.
Format: Article
Language:English
Subjects:
Bacterial adherence
Bacterial Adhesion - drug effects
Dentistry
Dimethicone copolyol
Durapatite
Humans
Maleates - pharmacology
Microbial Sensitivity Tests
Oral microflora
Poloxalene - pharmacology
Polyethylenes - pharmacology
Polyoxypropylene/polyoxyethylene block copolymer
PVM/MA copolymer
Saliva - microbiology
Simethicone - pharmacology
Streptococcus sanguis
Streptococcus sanguis - drug effects
Streptococcus sanguis - physiology
Toothpastes - chemistry
Toothpastes - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A microtitre assay has been developed using hydroxyapatite-coated wells and Streptococcus sanguis NCTC 10904 at 10 7 cells per ml. A number of models representing toothpaste and mouthwash usage were adopted to detect the anti-adherent efficacy of a polyvinylmethylether maleic acid copolymer (PVM/MA), polyoxypropylene/polyoxyethylene block copolymer (PO/EO), two casein-derived peptides and selected silicones. The results not only confirmed the anti-adherence property of the selected components but also indicated possible molecular interactions leading to the observed performance. To account for the diversity of oral microbial cells in vivo, a further testing system was developed. This involved submerging a hydroxyapatite disc in a mixed culture of human salivary microbial cells, and exposing it to different treatments using the active component either in an aqueous dispersion or in a toothpaste. The effect of toothpastes containing PO/EO, dimethicone copoyol or PVM/MA was investigated over a 4-h incubation with microflora. These tests showed that in a toothpaste formulation the anti-adherent efficacy may be reduced when compared with an aqueous dispersion containing the same or nearly the same concentration of the active component.
ISSN:0003-9969
1879-1506
DOI:10.1016/S0003-9969(00)00105-9