Investigation of bacterial spore structure by high resolution solid-state nuclear magnetic resonance spectroscopy and transmission electron microscopy

High resolution solid-state nuclear magnetic resonance spectroscopy (NMR) in combination with transmission electron microscopy (TEM) of spores of Bacillus cereus, an outer coatless mutant B. subtilis 322, an inner coatless mutant B. subtilis 325 and of germinated spores of B. subtilis CMCC 604 were...

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Bibliographic Details
Published in:International journal of food microbiology 2001-01, Vol.63 (1), p.35-50
Main Authors: Leuschner, Renata G.K, Lillford, Peter J
Format: Article
Language:English
Subjects:
Bacillus cereus
Bacillus cereus - ultrastructure
Bacillus subtilis
Bacillus subtilis - ultrastructure
Biological and medical sciences
dipicolinic acid
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Heat activation
Magnetic Resonance Spectroscopy - methods
Microscopy, Electron - methods
Mutation
Nuclear magnetic resonance
Picolinic Acids - chemistry
Spores
Spores, Bacterial - physiology
Spores, Bacterial - ultrastructure
Transmission electron microscopy
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Summary:High resolution solid-state nuclear magnetic resonance spectroscopy (NMR) in combination with transmission electron microscopy (TEM) of spores of Bacillus cereus, an outer coatless mutant B. subtilis 322, an inner coatless mutant B. subtilis 325 and of germinated spores of B. subtilis CMCC 604 were carried out. Structural differences in the coats, mainly protein of spores were reflected by NMR spectra which indicated also differences in molecular mobility of carbohydrates which was partially attributed to the cortex. Dipicolinic acid (DPA) of spores of B. cereus displayed a high degree of solid state order and may be crystalline. Heat activation was studied on spores of B. subtilis 357 lux+ and revealed a structural change when analysed by TEM but this was not associated with increases in molecular mobility since no effects were measured by NMR.
ISSN:0168-1605
1879-3460
DOI:10.1016/S0168-1605(00)00396-2