Precision of micellar electrokinetic capillary chromatography in the determination of seven antibiotics in pharmaceuticals and feedstuffs

Validation of analytical procedures is important for their efficient and reliable application. The International Conference on Harmonisation (ICH), Food and Drug Administration (FDA) and pharmacopoeia guidelines achieved a great deal in harmonising the definitions of the required validation characte...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 2007-06, Vol.594 (1), p.119-127
Main Authors: Injac, Rade, Kocevar, Nina, Kreft, Samo
Format: Article
Language:English
Subjects:
Analytical chemistry
Animal Feed - analysis
Anti-Bacterial Agents - analysis
Antibiotics
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Micellar Electrokinetic Capillary - methods
Exact sciences and technology
Feedstuffes
General, instrumentation
Micellar electrokinetic capillary chromatography
Other chromatographic methods
Pharmaceutical Preparations - chemistry
Pharmaceuticals
Precision
Reproducibility of Results
Software
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c412t-586a4200d092e3a1e09ba8bf8cfdb711303df9d356fb3704d12982d934201b1c3
cites cdi_FETCH-LOGICAL-c412t-586a4200d092e3a1e09ba8bf8cfdb711303df9d356fb3704d12982d934201b1c3
container_end_page 127
container_issue 1
container_start_page 119
container_title Analytica chimica acta
container_volume 594
creator Injac, Rade
Kocevar, Nina
Kreft, Samo
description Validation of analytical procedures is important for their efficient and reliable application. The International Conference on Harmonisation (ICH), Food and Drug Administration (FDA) and pharmacopoeia guidelines achieved a great deal in harmonising the definitions of the required validation characteristics. It is well known that poor reproducibility limits the practical implementation of capillary electrophoresis (CE). A precision study on four different MEKC methods was performed with 11 samples, containing seven antibiotics, by two analysts, in few days, on two capillary electrophoresis instruments. Five pharmaceutical preparations and three animal feeds were used. Precision was statistically analysed using migration time, peak area and height of each compound, as well as electroosmotic front (EOF). In 25 of 31 cases, the reproducibility of peak area, peak height and migration time was good (
doi_str_mv 10.1016/j.aca.2007.05.003
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70609899</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S000326700700863X</els_id><sourcerecordid>70609899</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-586a4200d092e3a1e09ba8bf8cfdb711303df9d356fb3704d12982d934201b1c3</originalsourceid><addsrcrecordid>eNqFkc9u1DAQxi0EotvCA3BBvsAty9jOP4sTqgpFqgQHOFuOPWa9bOJgO5X6CLw1DhupNzhZ_uY3n2bmI-QVgz0D1r477rXRew7Q7aHZA4gnZMf6TlS14PVTsoMiVbzt4IJcpnQsX86gfk4uWNe0IKTYkd9fIxqffJhocHT0Bk8nHSme0OQYfvoJszfU6Nmv-gM1hxhGncOPqOfDA_UTzQekFjPG0U86b0YJ73Giesp-8KE4pJWcDzqO2uBSBH1KpWypQ7QpL86lF-SZKyq-3N4r8v3jzbfr2-ruy6fP1x_uKlMznqumb3VdVrYgOQrNEOSg-8H1xtmhY0yAsE5a0bRuEB3UlnHZcytFaWIDM-KKvD37zjH8WjBlNfr0d-0Jw5JUBy3IXsr_glzKvuWyLiA7gyaGlCI6NUc_lmspBmoNSh1VCUqtQSloVEml9LzezJdhRPvYsSVTgDcboFO5lot6Kjk9cn3PuYB1yvdnDsvN7j1GlYzHyaD1JdmsbPD_GOMP5hGzKQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>29986294</pqid></control><display><type>article</type><title>Precision of micellar electrokinetic capillary chromatography in the determination of seven antibiotics in pharmaceuticals and feedstuffs</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Injac, Rade ; Kocevar, Nina ; Kreft, Samo</creator><creatorcontrib>Injac, Rade ; Kocevar, Nina ; Kreft, Samo</creatorcontrib><description>Validation of analytical procedures is important for their efficient and reliable application. The International Conference on Harmonisation (ICH), Food and Drug Administration (FDA) and pharmacopoeia guidelines achieved a great deal in harmonising the definitions of the required validation characteristics. It is well known that poor reproducibility limits the practical implementation of capillary electrophoresis (CE). A precision study on four different MEKC methods was performed with 11 samples, containing seven antibiotics, by two analysts, in few days, on two capillary electrophoresis instruments. Five pharmaceutical preparations and three animal feeds were used. Precision was statistically analysed using migration time, peak area and height of each compound, as well as electroosmotic front (EOF). In 25 of 31 cases, the reproducibility of peak area, peak height and migration time was good (&lt;5%). In most cases the reproducibility of peak area was much better than the reproducibility of peak height. The worst reproducibility that we observed was 12.7% for peak height and 7.6% for peak area.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2007.05.003</identifier><identifier>PMID: 17560393</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Animal Feed - analysis ; Anti-Bacterial Agents - analysis ; Antibiotics ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, Micellar Electrokinetic Capillary - methods ; Exact sciences and technology ; Feedstuffes ; General, instrumentation ; Micellar electrokinetic capillary chromatography ; Other chromatographic methods ; Pharmaceutical Preparations - chemistry ; Pharmaceuticals ; Precision ; Reproducibility of Results ; Software</subject><ispartof>Analytica chimica acta, 2007-06, Vol.594 (1), p.119-127</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-586a4200d092e3a1e09ba8bf8cfdb711303df9d356fb3704d12982d934201b1c3</citedby><cites>FETCH-LOGICAL-c412t-586a4200d092e3a1e09ba8bf8cfdb711303df9d356fb3704d12982d934201b1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=18822309$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17560393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Injac, Rade</creatorcontrib><creatorcontrib>Kocevar, Nina</creatorcontrib><creatorcontrib>Kreft, Samo</creatorcontrib><title>Precision of micellar electrokinetic capillary chromatography in the determination of seven antibiotics in pharmaceuticals and feedstuffs</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>Validation of analytical procedures is important for their efficient and reliable application. The International Conference on Harmonisation (ICH), Food and Drug Administration (FDA) and pharmacopoeia guidelines achieved a great deal in harmonising the definitions of the required validation characteristics. It is well known that poor reproducibility limits the practical implementation of capillary electrophoresis (CE). A precision study on four different MEKC methods was performed with 11 samples, containing seven antibiotics, by two analysts, in few days, on two capillary electrophoresis instruments. Five pharmaceutical preparations and three animal feeds were used. Precision was statistically analysed using migration time, peak area and height of each compound, as well as electroosmotic front (EOF). In 25 of 31 cases, the reproducibility of peak area, peak height and migration time was good (&lt;5%). In most cases the reproducibility of peak area was much better than the reproducibility of peak height. The worst reproducibility that we observed was 12.7% for peak height and 7.6% for peak area.</description><subject>Analytical chemistry</subject><subject>Animal Feed - analysis</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Antibiotics</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Micellar Electrokinetic Capillary - methods</subject><subject>Exact sciences and technology</subject><subject>Feedstuffes</subject><subject>General, instrumentation</subject><subject>Micellar electrokinetic capillary chromatography</subject><subject>Other chromatographic methods</subject><subject>Pharmaceutical Preparations - chemistry</subject><subject>Pharmaceuticals</subject><subject>Precision</subject><subject>Reproducibility of Results</subject><subject>Software</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxi0EotvCA3BBvsAty9jOP4sTqgpFqgQHOFuOPWa9bOJgO5X6CLw1DhupNzhZ_uY3n2bmI-QVgz0D1r477rXRew7Q7aHZA4gnZMf6TlS14PVTsoMiVbzt4IJcpnQsX86gfk4uWNe0IKTYkd9fIxqffJhocHT0Bk8nHSme0OQYfvoJszfU6Nmv-gM1hxhGncOPqOfDA_UTzQekFjPG0U86b0YJ73Giesp-8KE4pJWcDzqO2uBSBH1KpWypQ7QpL86lF-SZKyq-3N4r8v3jzbfr2-ruy6fP1x_uKlMznqumb3VdVrYgOQrNEOSg-8H1xtmhY0yAsE5a0bRuEB3UlnHZcytFaWIDM-KKvD37zjH8WjBlNfr0d-0Jw5JUBy3IXsr_glzKvuWyLiA7gyaGlCI6NUc_lmspBmoNSh1VCUqtQSloVEml9LzezJdhRPvYsSVTgDcboFO5lot6Kjk9cn3PuYB1yvdnDsvN7j1GlYzHyaD1JdmsbPD_GOMP5hGzKQ</recordid><startdate>20070626</startdate><enddate>20070626</enddate><creator>Injac, Rade</creator><creator>Kocevar, Nina</creator><creator>Kreft, Samo</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20070626</creationdate><title>Precision of micellar electrokinetic capillary chromatography in the determination of seven antibiotics in pharmaceuticals and feedstuffs</title><author>Injac, Rade ; Kocevar, Nina ; Kreft, Samo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-586a4200d092e3a1e09ba8bf8cfdb711303df9d356fb3704d12982d934201b1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analytical chemistry</topic><topic>Animal Feed - analysis</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Antibiotics</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, Micellar Electrokinetic Capillary - methods</topic><topic>Exact sciences and technology</topic><topic>Feedstuffes</topic><topic>General, instrumentation</topic><topic>Micellar electrokinetic capillary chromatography</topic><topic>Other chromatographic methods</topic><topic>Pharmaceutical Preparations - chemistry</topic><topic>Pharmaceuticals</topic><topic>Precision</topic><topic>Reproducibility of Results</topic><topic>Software</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Injac, Rade</creatorcontrib><creatorcontrib>Kocevar, Nina</creatorcontrib><creatorcontrib>Kreft, Samo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Injac, Rade</au><au>Kocevar, Nina</au><au>Kreft, Samo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Precision of micellar electrokinetic capillary chromatography in the determination of seven antibiotics in pharmaceuticals and feedstuffs</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2007-06-26</date><risdate>2007</risdate><volume>594</volume><issue>1</issue><spage>119</spage><epage>127</epage><pages>119-127</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>Validation of analytical procedures is important for their efficient and reliable application. The International Conference on Harmonisation (ICH), Food and Drug Administration (FDA) and pharmacopoeia guidelines achieved a great deal in harmonising the definitions of the required validation characteristics. It is well known that poor reproducibility limits the practical implementation of capillary electrophoresis (CE). A precision study on four different MEKC methods was performed with 11 samples, containing seven antibiotics, by two analysts, in few days, on two capillary electrophoresis instruments. Five pharmaceutical preparations and three animal feeds were used. Precision was statistically analysed using migration time, peak area and height of each compound, as well as electroosmotic front (EOF). In 25 of 31 cases, the reproducibility of peak area, peak height and migration time was good (&lt;5%). In most cases the reproducibility of peak area was much better than the reproducibility of peak height. The worst reproducibility that we observed was 12.7% for peak height and 7.6% for peak area.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17560393</pmid><doi>10.1016/j.aca.2007.05.003</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-2670
ispartof Analytica chimica acta, 2007-06, Vol.594 (1), p.119-127
issn 0003-2670
1873-4324
language eng
recordid cdi_proquest_miscellaneous_70609899
source ScienceDirect Freedom Collection 2022-2024
subjects Analytical chemistry
Animal Feed - analysis
Anti-Bacterial Agents - analysis
Antibiotics
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Micellar Electrokinetic Capillary - methods
Exact sciences and technology
Feedstuffes
General, instrumentation
Micellar electrokinetic capillary chromatography
Other chromatographic methods
Pharmaceutical Preparations - chemistry
Pharmaceuticals
Precision
Reproducibility of Results
Software
title Precision of micellar electrokinetic capillary chromatography in the determination of seven antibiotics in pharmaceuticals and feedstuffs
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A14%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Precision%20of%20micellar%20electrokinetic%20capillary%20chromatography%20in%20the%20determination%20of%20seven%20antibiotics%20in%20pharmaceuticals%20and%20feedstuffs&rft.jtitle=Analytica%20chimica%20acta&rft.au=Injac,%20Rade&rft.date=2007-06-26&rft.volume=594&rft.issue=1&rft.spage=119&rft.epage=127&rft.pages=119-127&rft.issn=0003-2670&rft.eissn=1873-4324&rft.coden=ACACAM&rft_id=info:doi/10.1016/j.aca.2007.05.003&rft_dat=%3Cproquest_cross%3E70609899%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c412t-586a4200d092e3a1e09ba8bf8cfdb711303df9d356fb3704d12982d934201b1c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=29986294&rft_id=info:pmid/17560393&rfr_iscdi=true