The influence of intrinsic coagulation pathway on blood platelets activation by oxidized cellulose

Oxidized cellulose is an effective haemostat that works naturally to aid in blood coagulation. The mechanism of its action is not very well understood. Little effect on blood coagulation, but a pronounce decrease in platelet count has been reported upon the addition of the oxidized cellulose to the...

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Published in:Journal of biomedical materials research. Part A 2007-08, Vol.82A (2), p.274-280
Main Authors: Křížová, P., Mášová, L., Suttnar, J., Salaj, P., Dyr, J.E., Homola, J., Pecka, M.
Format: Article
Language:English
Subjects:
Biocompatible Materials - pharmacology
Blood Coagulation - drug effects
Blood Coagulation - physiology
blood platelets
cellulose
Cellulose, Oxidized - pharmacology
coagulation factor VIII
coagulation factor VIII, IX, XII, XIII
Coagulation Protein Disorders - blood
Hemostatics - pharmacology
Humans
In Vitro Techniques
Materials Testing
Platelet Activation - drug effects
Platelet Activation - physiology
Platelet Aggregation - drug effects
serotonin
Serotonin - blood
Serotonin - secretion
XII
XIII
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Summary:Oxidized cellulose is an effective haemostat that works naturally to aid in blood coagulation. The mechanism of its action is not very well understood. Little effect on blood coagulation, but a pronounce decrease in platelet count has been reported upon the addition of the oxidized cellulose to the whole blood. As a marker of platelet activation and aggregation we used serotonin release reaction and turbidity changes in time. We found that oxidized cellulose did not activate washed platelets reconstituted in plasma‐free medium or plasma‐free medium with fibrinogen; no reduction of platelet count was observed. Serotonin release in platelet‐rich plasma incubated with oxidized cellulose started in the range from 5 to 10 min. Serotonin release from platelets reconstituted in plasma deficient in either coagulation factor V, VIII, IX, or XII was delayed. Blood platelets activation by oxidized cellulose requires calcium ions present in dispersion of oxidized cellulose. Factor XIII deficiency had no influence on blood platelets activation by oxidized cellulose. Our results clearly indicate the significance of intrinsic coagulation pathway activation on blood platelets activation by oxidized cellulose and so indirectly on the haemostyptic effect of oxidized cellulose. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.31060