Loading…
Gastrodia elata Blume protects against stress-induced gastric mucosal lesions in mice
Gastrodia elata Blume (GEB) is a traditional herbal plant that has been used in Asian countries for centuries as an anticonvulsant, analgesic, and also as a sedative for treating general paralysis, epilepsy, vertigo, and tetanus. Although numerous reports have addressed the effects of GEB against de...
Saved in:
Published in: | International journal of molecular medicine 2007-08, Vol.20 (2), p.209-215 |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Gastrodia elata Blume (GEB) is a traditional herbal plant that has been
used in Asian countries for centuries as an anticonvulsant, analgesic, and also
as a sedative for treating general paralysis, epilepsy, vertigo, and tetanus.
Although numerous reports have addressed the effects of GEB against degenerative
diseases, no previous study has examined the possible gastroprotective effects
of GEB. Here, we examined the effects of pretreatment with GEB (0.02 ml/g, p.o.)
in a mouse water immersion restraint (WIR) stress-induced gastric lesion model.
Our results revealed that mice pretreated with GEB had significantly fewer gastric
lesions than their respective controls. Moreover, GEB-treated mice showed significant
decreases in serum and gastric nitric oxide (NO) levels to 50 and 28%, respectively.
To examine one possible mechanism underlying this effect, we used reverse transcription-polymerase
chain reaction (RT-PCR) to examine NOS mRNA expression in gastric lesion tissues.
Our results revealed that the mRNA expression of inducible nitric oxide synthase
(iNOS) was reduced by ≈50% in GEB-pretreated mice versus the controls, whereas
the mRNA expression levels of endothelial nitric oxide synthase (eNOS) and neuronal
nitric oxide synthase (nNOS) remained unchanged. These findings collectively suggest
that GEB significantly protects the gastric mucosa against WIR-induced gastric
damage, at least in part by decreasing NO levels via suppression of iNOS mRNA
expression. |
---|---|
ISSN: | 1107-3756 1791-244X |
DOI: | 10.3892/ijmm.20.2.209 |