A random model for mapping imprinted quantitative trait loci in a structured pedigree: An implication for mapping canine hip dysplasia

• Published in: Genomics (San Diego, Calif.) Calif.), 2007-08, Vol.90 (2), p.276-284
• Main Authors: Liu, Tian, Todhunter, Rory J., Wu, Song, Hou, Wei, Mateescu, Raluca, Zhang, Zhiwu, Burton-Wurster, Nancy I., Acland, Gregory M., Lust, George, Wu, Rongling
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Canine hip dysplasia; Diseases of the osteoarticular system; Dogs; Fundamental and applied biological sciences. Psychology; Genes. Genome; Genetic Linkage; Genetics of eukaryotes. Biological and molecular evolution; Genomic Imprinting; Hip Dysplasia, Canine - genetics; Imprinting quantitative trait loci; Malformations and congenital and or hereditary diseases involving bones. Joint deformations; Medical sciences; Models, Statistical; Molecular and cellular biology; Molecular genetics; Parent-of-origin effect; Pedigree; Quantitative Trait Loci; Random-effect model
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2007.04.004

Genetic imprinting may have played a more notable role in shaping embryonic development of plants, animals, and humans than previously appreciated. Quantitative trait loci that are imprinted ( iQTL) exert monoallelic effects, depending on the parent of origin, which is an exception to the laws of Mendelian genetics. In this article, we present a modified random effect-based mapping model to use in a genome-wide scan for the distribution of iQTL that contribute to genetic variance for a complex trait in a structured pedigree. This model, implemented with the maximum likelihood method, capitalizes on a network of relatedness for maternally and paternally derived alleles through identical-by-descent sharing, thus allowing for the discrimination of the genetic variances due to alleles derived from maternal and paternal parents. The model was employed to map iQTL responsible for canine hip dysplasia in a multihierarchical canine pedigree, founded with seven greyhounds and six Labrador retrievers. Of eight significant QTL detected, three, located on CFA1, CFA8, and CF28, were found to trigger significant parent-of-origin effects on the age of femoral capital ossification measured at the left and right hips of a canine. The detected iQTL provide important candidate regions for fine-mapping of imprinted genes and for studying their structure and function in the control of complex traits.