Ribosomal distributions in axons of mammalian myelinated fibers

The distribution of ribosomes and polysomes in uninjured myelinated axons of rat sciatic nerve was analyzed. Ribosomes were identified by immunocytochemistry at the light and electron microscopic levels. A polyclonal antibody developed against ribosomes recognized both rRNA and ribosomal proteins. T...

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Bibliographic Details
Published in:Journal of neuroscience research 2007-08, Vol.85 (10), p.2087-2098
Main Authors: Kun, Alejandra, Otero, Leonardo, Sotelo-Silveira, José R., Sotelo, José R.
Format: Article
Language:English
Subjects:
Animals
anti-ribosome antibody
Axons - ultrastructure
Immunohistochemistry
Microscopy, Electron
myelinated axons
Nerve Fibers, Myelinated - ultrastructure
Rats
Ribosomal Proteins - metabolism
Ribosomes - genetics
Ribosomes - metabolism
Ribosomes - ultrastructure
RNA, Ribosomal - metabolism
RNPs
Sciatic Nerve - ultrastructure
Tissue Distribution
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Summary:The distribution of ribosomes and polysomes in uninjured myelinated axons of rat sciatic nerve was analyzed. Ribosomes were identified by immunocytochemistry at the light and electron microscopic levels. A polyclonal antibody developed against ribosomes recognized both rRNA and ribosomal proteins. The distribution of the immunoreaction product was similar to that obtained with human anti‐ribosomal P protein. The immunoreaction product distributions were of two types in axons: 1) periodic localization in the cortical region of axoplasm that appeared as a compact structural aggregate, consistent with that described as a periaxoplasmic ribosomal plaques (PARP) domain (Koenig et al. [2000] J. Neurosci. 20:8390–8400), and 2) scattered small immuno‐reactive clusters of varying sizes (RNP) within the central core of the axon. The latter observation suggested the possibility that RNP‐like particles could be associated with the axonal transport system and in transit. Immunoreaction product was also associated with a novel structural inclusion, possibly multi‐vesicular in makeup that was located in the axon and at the myelin‐axon interface, and visible at the light and EM levels. The potential significance of this structural peculiarity is considered. © 2007 Wiley‐Liss, Inc.
ISSN:0360-4012
1097-4547
DOI:10.1002/jnr.21340