Sensitization and Activation of Intracranial Meningeal Nociceptors by Mast Cell Mediators

• Published in: The Journal of pharmacology and experimental therapeutics 2007-08, Vol.322 (2), p.806-812
• Main Authors: Zhang, Xi-Chun, Strassman, Andrew M, Burstein, Rami, Levy, Dan
• Format: Article
• Language: English
• Subjects: Action Potentials - drug effects; Animals; Dose-Response Relationship, Drug; Electrophysiology; Epoprostenol - metabolism; Epoprostenol - pharmacology; Histamine - metabolism; Histamine - pharmacology; Leukotriene C4 - pharmacology; Male; Mast Cells - metabolism; Meninges - cytology; Meninges - drug effects; Meninges - physiology; Nociceptors - drug effects; Nociceptors - physiology; Prostaglandin D2 - pharmacology; Rats; Rats, Sprague-Dawley; Serotonin - metabolism; Serotonin - pharmacology; Stress, Mechanical
• ISSN: 0022-3565; 1521-0103
• DOI: 10.1124/jpet.107.123745

Intracranial headaches such as migraine are thought to result from activation of sensory trigeminal pain neurons that supply intracranial blood vessels and the meninges, also known as meningeal nociceptors. Although the mechanism underlying the triggering of such activation is not completely understood, our previous work indicates that the local activation of the inflammatory dural mast cells can provoke a persistent sensitization of meningeal nociceptors. Given the potential importance of mast cells to the pain of migraine it is important to understand which mast cell-derived mediators interact with meningeal nociceptors to promote their activation and sensitization. In the present study, we have used in vivo electrophysiological single-unit recording of meningeal nociceptors in the trigeminal ganglion of anesthetized rats to examine the effect of a number of mast cell mediators on the activity level and mechanosensitivity of meningeal nociceptors. We have found that that serotonin (5-HT), prostaglandin I 2 (PGI 2 ), and to a lesser extent histamine can promote a robust sensitization and activation of meningeal nociceptors, whereas the inflammatory eicosanoids PGD 2 and leukotriene C 4 are largely ineffective. We propose that dural mast cells could promote headache by releasing 5-HT, PGI 2 , and histamine.