Separating DNA sequencing fragments without a sieving matrix

• Published in: Electrophoresis 1999-08, Vol.20 (12), p.2501-2509
• Main Authors: Ren, Hongji, Karger, Achim E., Oaks, Frank, Menchen, Steve, Slater, Gary W., Drouin, Guy
• Format: Article
• Language: English
• Subjects: Buffers; Capillary electrophoresis; Diffusion; DNA - isolation & purification; DNA separations; Electrophoresis, Capillary - methods; Hydrogen-Ion Concentration; Sequence Analysis, DNA; Sequencing; Streptavidin - isolation & purification
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/(SICI)1522-2683(19990801)20:12<2501::AID-ELPS2501>3.0.CO;2-H

The possibility of separating appropriately labeled DNA fragments using free‐flow capillary electrophoresis was predicted a few years ago based on simple theoretical arguments. Free‐flow separation of double‒stranded DNA (dsDNA) fragments in the 100—1000 base range was later demonstrated using a streptavidin label. In this article, we now report that end‒labeled free‒flow electrophoresis (ELFSE) can also be used to sequence single‒stranded DNA (ssDNA). The first 100 bases of a DNA sequencing reaction were read without any sieving matrix when fractionated streptavidin was added to the 5′‒end of the ssDNA fragments. These separations required only 18 min and did not require coated capillaries. An analysis of the results indicates that sample injection, analyte‒wall interactions and thermal diffusion are the limiting factors at this time. Extrapolating from our data, we predict that several hundred bases could be sequenced in less than 30 min with the proper conditions. ELFSE thus offers an attractive potential alternative to polymer solutions for DNA sequencing in capillaries and microchips.