Evaluation of whole genome amplification methods using postmortem brain samples

The importance of examining genomic DNA derived from human brain has been highlighted by recent findings such as the possible link between DNA methylation and behavior or mental disorders, as well as the possible genomic differences between neurons from the same individual caused by transposons and...

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Bibliographic Details
Published in:Journal of neuroscience methods 2007-09, Vol.165 (1), p.104-110
Main Authors: Iwamoto, Kazuya, Ueda, Junko, Nakano, Yoko, Bundo, Miki, Ukai, Wataru, Hashimoto, Eri, Saito, Toshikazu, Kato, Tadafumi
Format: Article
Language:English
Subjects:
Autopsy
Brain
DNA - isolation & purification
Genome, Human
Humans
Nucleic Acid Amplification Techniques
phi29 DNA polymerase
Polymerase Chain Reaction
Postmortem brain
Single nucleotide polymorphisms
Whole genome amplification
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Summary:The importance of examining genomic DNA derived from human brain has been highlighted by recent findings such as the possible link between DNA methylation and behavior or mental disorders, as well as the possible genomic differences between neurons from the same individual caused by transposons and aneuploidy. Consequently, obtaining a sufficient amount of genomic DNA derived from human brain is a critical issue for further research. Whole genome amplification (WGA) methods, by which genomic DNA is typically amplified on the order of 10 4–10 6, will be a valuable tool for providing a sufficient amount of DNA for various molecular genetic studies. Here we evaluated three methods, including both PCR-based and non-PCR based WGA, as well as DNA extraction methods using frozen postmortem brain tissue. We found that WGA products from postmortem brains can be used in molecular genetic analysis, if a particular protocol for DNA extraction is used, and the most appropriate method for WGA depends on the state of the genomic DNA to be amplified.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2007.05.029