Validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis

• Published in: Veterinary microbiology 1999-08, Vol.68 (3), p.245-253
• Main Authors: Nielsen, K, Gall, D, Smith, P, Vigliocco, A, Perez, B, Samartino, L, Nicoletti, P, Dajer, A, Elzer, P, Enright, F
• Format: Article
• Language: English
• Subjects: Agglutination Tests - veterinary; Animal bacterial diseases; Animals; Antibodies, Bacterial - blood; Bacterial diseases; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Brucella - immunology; Brucella - isolation & purification; Brucella sp; BRUCELLA SUIS; Brucellosis - diagnosis; Brucellosis - immunology; Brucellosis - veterinary; CERDO; Complement Fixation Tests - veterinary; Diagnosis; Enzyme immunoassay; Enzyme-Linked Immunosorbent Assay - veterinary; FLUORESCENCE; Fluorescence polarization assay; Fluorescence Polarization Immunoassay - veterinary; FLUORESCENCIA; Fundamental and applied biological sciences. Psychology; IMMUNODIAGNOSIS; IMMUNODIAGNOSTIC; IMMUNOLOGICAL TECHNIQUES; IMMUNOLOGIE; IMMUNOLOGY; Infectious diseases; INMUNODIAGNOSTICO; INMUNOLOGIA; Medical sciences; Microbiology; PORCIN; Porcine brucellosis; ROC Curve; Sensitivity and Specificity; Serology; SWINE; Swine Diseases - diagnosis; Swine Diseases - immunology; Swine Diseases - microbiology; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/S0378-1135(99)00077-2

Sera from Canadian pigs (brucellosis free, n = 14 037) and sera from pigs infected with Brucella suis ( n = 401) were tested by the buffered antigen plate agglutination test, the complement fixation test, an indirect and a competitive enzyme immunoassay and a fluorescence polarization assay. The results were analysed and assay sensitivity and specificity estimates were calculated. The sensitivity and specificity of the tests were as follows: the buffered antigen plate agglutination test, 77.1 and 96.9%; the complement fixation test (considering anticomplementary sera as negative), 93.3 and 95.5%; the complement fixation test (considering anticomplementary sera as positive), 58.1 and 99.9%; the indirect enzyme immunoassay, 94.0 and 97.9%; the competitive enzyme immunoassay, 90.8 and 96.6%; and the fluorescence polarization assay, 93.5 and 97.2%; respectively. It was concluded that the fluorescence polarization assay was a valuable asset to the diagnosis of porcine brucellosis because of its accuracy, ease of performance and relative cost.