Mammalian Oocyte Activation by the Synergistic Action of Discrete Sperm Head Components: Induction of Calcium Transients and Involvement of Proteolysis

• Published in: Developmental biology 2000-01, Vol.217 (2), p.386-393
• Main Authors: Perry, Anthony C.F., Wakayama, Teruhiko, Cooke, Ian M., Yanagimachi, Ryuzo
• Format: Article
• Language: English
• Subjects: Animals; calcium; Calcium Signaling; Female; Male; Meiosis; Metaphase; Mice; Models, Biological; mouse; oocyte activation; Oocytes - physiology; Periodicity; Proteins - metabolism; proteolysis; Serine Endopeptidases - metabolism; Serine Proteinase Inhibitors - metabolism; sperm; Sperm Head - physiology; Sperm-Ovum Interactions - physiology
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1006/dbio.1999.9552

Sperm-borne oocyte-activating factor (SOAF) elicits activation sufficient for full development and originates from sperm head submembrane matrices. SOAF comprises discrete, heat-sensitive and -stable components (referred to here respectively as SOAF-I and -II) which are each necessary but not sufficient to activate oocytes. The heat-sensitive SOAF component, SOAF-Im, becomes solubilized from the perinuclear matrix under reducing conditions (the SOAF transition) to generate SOAF-Is. Although calcium transients likely play an important role in oocyte activation at fertilization, the question is open as to whether demembranated heads or SOAF-Is and/or SOAF-II can induce calcium transients. We now report that injection of demembranated sperm heads into mouse oocytes efficiently induced Ca2+ oscillations. When injected independently, SOAF-Is and demembranated heads heated to 48°C failed to generate Ca2+ oscillations. However, co-injection of SOAF-Is and 48°C-heated heads induced oscillations, mirroring their synergistic ability to activate oocytes. This suggests that SOAF-mediated activation proceeds via pathways resembling those at fertilization and provides the first direct evidence that multiple sperm components are required to induce Ca2+ oscillations. We probed the SOAF-Is liberation at the center of this activation and show that in vitro it was sensitive to a profile of serine protease inhibitors. These findings support a model in which mammalian oocyte activation, including the induction of calcium transients, involves proteolytic processing of SOAF from sperm head submembrane compartments.