Fluorescent strip-lights as a source of error in tissue reflectance spectroscopy

Tissue reflectance spectroscopy is a widely used technique for measuring the blood content of the skin. Fluorescent strip-lighting of a type in widespread use (Osram L36 W/23 Gelbweiss-white) is a potential source of misleading artefacts in tissue reflectance spectroscopy. The fluorescent light sign...

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Bibliographic Details
Published in:Medical engineering & physics 1999-05, Vol.21 (4), p.241-245
Main Authors: Bain, D.S, Ferguson-Pell, M.W
Format: Article
Language:English
Subjects:
Algorithms
Biological and medical sciences
Blood
Dermatology
Erythema
Fluorescence
Humans
In Vitro Techniques
Investigative techniques, diagnostic techniques (general aspects)
Light reflection
Lighting
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reflectance spectroscopy
Skin
Skin - blood supply
Skin - radiation effects
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
Spectrometry, Fluorescence - statistics & numerical data
Spectrophotometry
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Summary:Tissue reflectance spectroscopy is a widely used technique for measuring the blood content of the skin. Fluorescent strip-lighting of a type in widespread use (Osram L36 W/23 Gelbweiss-white) is a potential source of misleading artefacts in tissue reflectance spectroscopy. The fluorescent light signal has successive peaks and troughs in the 520–580 nm range which closely emulate the inverse of the reflected signal of white light modulated by blood. The presence of fluorescent light in the reflected signal cancels some of the blood signature. Conversely, the presence of fluorescent light in the white reference augments the true blood reflected signal. The marked similarity of the resultant artefact blood spectrum to the true blood spectrum is a hazard, as the presence of the artefact may go undetected.
ISSN:1350-4533
1873-4030
DOI:10.1016/S1350-4533(99)00044-2