Soybean Isoflavones Trigger a Calcium Influx in Phytophthora sojae

Both the motile zoospores and the hyphal germ tubes of Phytophthora sojae respond chemotropically to the soybean isoflavones daidzein and genistein. The role of Ca2+ in the cellular response to these host signals was investigated by using X-ray microanalysis of cells to monitor net changes in cellul...

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Bibliographic Details
Published in:Fungal genetics and biology 1999-10, Vol.28 (1), p.6-11
Main Authors: Connolly, Mary S, Williams, Nicole, Heckman, Carol A, Morris, Paul F
Format: Article
Language:English
Subjects:
caffeine
calcium
Calcium - agonists
Calcium - metabolism
calcium stores
cell differentiation
Chemotaxis
Culture Media - chemistry
cysts
daidzein
development
Electron Probe Microanalysis
encystment
genistein
Genistein - pharmacology
Glycine max
Glycine max - chemistry
hyphae
hyphal germ tubes
hyphal germlings
inorganic ions
ion transport
Isoflavones - pharmacology
Phytophthora
Phytophthora - drug effects
Phytophthora - physiology
Phytophthora sojae
Spores - chemistry
Spores - growth & development
stimulation
X-ray microanalysis
zoospores
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Summary:Both the motile zoospores and the hyphal germ tubes of Phytophthora sojae respond chemotropically to the soybean isoflavones daidzein and genistein. The role of Ca2+ in the cellular response to these host signals was investigated by using X-ray microanalysis of cells to monitor net changes in cellular levels of Ca2+ and by quantifying the effects of exogenous Ca2+ and daidzein on the developmental fate of encysted zoospores. Confirmation that isoflavones trigger a net influx of Ca2+ into the cell was demonstrated by X-ray microanalysis of individual encysted zoospores. Zoospores exposed to 10 mM Ca2+ and 1 μM daidzein at the time of encystment formed cysts that contained more Ca2+ than zoospores exposed to Ca2+ alone. The magnitude of internal Ca2+ stores appears to be a determining factor affecting the developmental fate of P. sojae cysts.
ISSN:1087-1845
1096-0937
DOI:10.1006/fgbi.1999.1148