The Amino-Terminal 1−185 Domain of ApoE Promotes the Clearance of Lipoprotein Remnants in Vivo. The Carboxy-Terminal Domain Is Required for Induction of Hyperlipidemia in Normal and ApoE-Deficient Mice

• Published in: Biochemistry (Easton) 2001-05, Vol.40 (20), p.6027-6035
• Main Authors: Kypreos, Kyriakos E, Morani, Pamela, van Dijk, Ko Willems, Havekes, Louis M, Zannis, Vassilis I
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Adenovirus; Animals; Apolipoprotein E3; Apolipoprotein E4; Apolipoproteins E - biosynthesis; Apolipoproteins E - deficiency; Apolipoproteins E - genetics; Apolipoproteins E - metabolism; Apolipoproteins E - physiology; Biological Transport, Active - genetics; Chromatography, High Pressure Liquid; Female; Gene Deletion; Genetic Vectors - chemistry; Genetic Vectors - metabolism; Humans; Hypercholesterolemia - blood; Hypercholesterolemia - etiology; Hypercholesterolemia - genetics; Hyperlipidemias - blood; Hyperlipidemias - etiology; Hyperlipidemias - genetics; Hypertriglyceridemia - blood; Hypertriglyceridemia - etiology; Hypertriglyceridemia - genetics; Lipoproteins - blood; Lipoproteins - metabolism; Lipoproteins, VLDL - metabolism; Liver - metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Peptide Fragments - genetics; Peptide Fragments - physiology; Protein Structure, Tertiary - genetics; RNA, Messenger - metabolism; Triglycerides - metabolism; Tumor Cells, Cultured
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi002414a

Apolipoprotein E (apoE) promotes receptor-mediated catabolism of apoE-containing lipoprotein remnants. Impairments in remnant clearance are associated with type III hyperlipoproteinemia and premature atherosclerosis. In humans, apoE plasma levels correlate with plasma triglyceride levels, suggesting that excess apoE may also affect plasma triglyceride levels. We have used adenovirus-mediated gene transfer in mice to map the domains of apoE required for cholesterol and triglyceride clearance, in vivo. Adenovirus expressing apoE3 and apoE4 at doses of (1−2) × 109 pfu increased plasma cholesterol and triglyceride levels in normal C57BL6 mice and failed to normalize the high cholesterol levels of apoE-deficient mice due to induction of hypertriglyceridemia. In contrast, an adenovirus expressing the truncated apoE 1−185 form normalized the cholesterol levels of E-/- mice and did not cause hypertriglyceridemia. Northern blot analysis of hepatic RNA from mice expressing the full-length and the truncated apoE forms showed comparable steady-state apoE mRNA levels of the full-length apoE forms that cause hyperlipidemia and the truncated apoE forms that do not cause hyperlipidemia. The findings suggest that the amino-terminal residues 1−185 of apoE are sufficient for the clearance of apoE-containing lipoprotein remnants by the liver, whereas domains of the carboxy-terminal one-third of apoE are required for apoE-induced hyperlipidemia.