Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins

Human Ro ribonucleoproteins (RNPs) are autoantigenic particles of unknown function(s) that consist of a 60-kDa protein (Ro60) associated with one hY RNA (hY1–5). Using a modified yeast three-hybrid system, named RNP interaction trap assay (RITA), we cloned a novel Ro RNP-binding protein (RoBPI), bas...

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Bibliographic Details
Published in:	RNA (Cambridge) 2000-01, Vol.6 (1), p.66-78, Article S1355838200990277
Main Authors:	BOUFFARD, PASCAL, BARBAR, ELIE, BRIÈRE, FRANCIS, BOIRE, GILLES
Format:	Article
Language:	English
Subjects:	Amino Acid Sequence Animals Base Sequence Blotting, Northern Carrier Proteins - genetics Carrier Proteins - metabolism Cell Extracts Cell Nucleus - metabolism Cloning, Molecular DNA-Binding Proteins expressed sequence tags Fluorescent Antibody Technique, Indirect HeLa Cells Humans Microscopy, Confocal Molecular Sequence Data Organ Specificity Polymerase Chain Reaction Protein Binding Rabbits Recombinant Proteins - metabolism Ribonucleoproteins - genetics Ribonucleoproteins - metabolism RNA-Binding Proteins Ro proteins Ro RNP-binding protein Yeasts
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