Plasminogen Binding Is Increased with Adipocyte Differentiation

The purpose of this study was to examine the role of the plasminogen system in the development of adipose tissue. Plasminogen binding capacity was determined in differentiated and undifferentiated cells from adipose tissue of plasminogen deficient mice and 3T3 cells, a well-characterized tissue cult...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-06, Vol.284 (2), p.389-394
Main Authors: Hoover-Plow, Jane, Yuen, Lawrence
Format: Article
Language:English
Subjects:
3T3 Cells
Adipocytes - cytology
Adipocytes - metabolism
Aminocaproic Acid - pharmacology
Animals
Cell Differentiation - physiology
Cells, Cultured
Dose-Response Relationship, Drug
Ligands
Lysine - pharmacology
mature fat cells
Mice
Mice, Knockout
plasminogen
Plasminogen - deficiency
Plasminogen - genetics
Plasminogen - metabolism
plasminogen deficient mice
plasminogen gene
Protein Binding - drug effects
Protein Binding - physiology
stromal cells
Stromal Cells - cytology
Stromal Cells - metabolism
Tranexamic Acid - pharmacology
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Summary:The purpose of this study was to examine the role of the plasminogen system in the development of adipose tissue. Plasminogen binding capacity was determined in differentiated and undifferentiated cells from adipose tissue of plasminogen deficient mice and 3T3 cells, a well-characterized tissue culture model. In 3T3 cells, plasminogen binding was fivefold higher in differentiated cells compared to the undifferentiated cells. Inhibition of binding by carboxyl-terminal lysine analogs was similar for the differentiated and undifferentiated cells with tranexamic acid > EACA > lysine. The binding of plasminogen was concentration-dependent and approaches saturation in the both cell types. The number of plasminogen binding sites was tenfold higher in the differentiated compared to the undifferentiated cells. In isolated mature fat cells and stromal cell cultures from mouse adipose tissue, plasminogen binding was also higher in the differentiated mature fat cells and differentiated stromal cells compared to undifferentiated stromal cells. Plasminogen binding was elevated in the differentiated cells from the Plg−/− mice compared to cells from the WT mice. These results suggest that the plasminogen system plays an important role in adipose tissue development.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4984