Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes

The objective of the present study was to understand the role of cortisol in the cellular stress response process in fish. Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintaine...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2001-06, Vol.129 (2-3), p.679-685
Main Authors: Sathiyaa, Ramesh, Campbell, Tracey, Vijayan, Mathilakath M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cells, Cultured
Cortisol
Fish
Gene Expression Regulation - drug effects
Glucocorticoid receptors
Heat shock protein
Hepatocyte primary cultures
Hepatocytes - cytology
Hepatocytes - drug effects
Hepatocytes - metabolism
HSP90 Heat-Shock Proteins - chemistry
HSP90 Heat-Shock Proteins - genetics
HSP90 mRNA
Hydrocortisone - pharmacology
Molecular Sequence Data
Oncorhynchus mykiss
Oncorhynchus mykiss - genetics
Rainbow trout
RNA, Messenger - genetics
RNA, Messenger - metabolism
Stress
Temperature shock
Time Factors
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Summary:The objective of the present study was to understand the role of cortisol in the cellular stress response process in fish. Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintained in primary culture. We also subjected these hepatocytes to heat shock (HS) in order to examine the role of cortisol on HS-induced HSP90 mRNA expression. A cDNA fragment of 500 bp was cloned from trout liver by reverse transcriptase- polymerase chain reaction (RT-PCR) with primers designed from the conserved regions of chinook salmon and zebrafish HSP90 cDNAs. The PCR product showed very high homology to chinook salmon (98%), zebrafish (84%) and human (77%) HSP90. Heat shock (+6°C) induced transient elevation in HSP90 mRNA in trout hepatocytes, peaking within 10-h post-HS, and remained elevated over a 24-h period. Cortisol did not modify the unstimulated expression of HSP90 mRNA, whereas the HS-induced HSP90 mRNA expression was attenuated in trout hepatocytes. Our results suggest that elevated plasma cortisol levels modulate the cellular stress response by affecting the transcription of HSP90 in fish.
ISSN:1096-4959
1879-1107
DOI:10.1016/S1096-4959(01)00373-6