Photodynamic Therapy Generates a Matrix Barrier to Invasive Vascular Cell Migration

ABSTRACTPhotodynamic therapy (PDT) inhibits experimental intimal hyperplasia. PDT results in complete vascular wall cell eradication with subsequent adventitia but minimal media repopulation. This study was designed to test the hypothesis that PDT alters the vascular wall matrix thereby inhibiting i...

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Bibliographic Details
Published in:Circulation research 2000-02, Vol.86 (3), p.334-340
Main Authors: Overhaus, Marcus, Heckenkamp, Joerg, Kossodo, Sylvie, Leszczynski, Dariusz, LaMuraglia, Glenn M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood Vessels - cytology
Blood Vessels - drug effects
Blood Vessels - physiology
Blotting, Western
Carotid Artery Injuries - pathology
Catheterization
Cattle
Cell Movement - physiology
Cells, Cultured
Diseases of the cardiovascular system
Electrophoresis, Gel, Two-Dimensional
Enzyme-Linked Immunosorbent Assay
Extracellular Matrix - physiology
Medical sciences
Photochemotherapy
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Rats
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Summary:ABSTRACTPhotodynamic therapy (PDT) inhibits experimental intimal hyperplasia. PDT results in complete vascular wall cell eradication with subsequent adventitia but minimal media repopulation. This study was designed to test the hypothesis that PDT alters the vascular wall matrix thereby inhibiting invasive cell migration, and as such, provides an important barrier mechanism to favorably alter the vascular injury response. Untreated smooth muscle cells (SMCs) and fibroblasts were seeded on control and PDT-treated (100 J/cm; photosensitizer was chloroaluminum-sulfonated phthalocyanine, 5 μg/mL) 3-dimensional collagen matrix gels. Invasive cell migration was temporally quantified by calibrated microscopy. Zymography and ELISA assessed SMC matrix metalloproteinase levels. Molecular changes of gel proteins and their susceptibility to collagenase were analyzed by SDS-PAGE and Western blot. Limited pepsin digestion and histology were used to assess the in vivo relevance of the model, using an established rat carotid artery model at 1 and 4 weeks after balloon injury and PDT. PDT of 3-dimensional matrix of gels led to a 52% reduction of invasive SMCs and to a 59% reduction of fibroblast migration (P
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.86.3.334