Immunohistochemical Localization of Modified C-Reactive Protein Antigen in Normal Vascular Tissue

Background: The prototypic acute phase reactant, C-reactive protein (CRP), is a serum soluble, cyclic pentameric protein, the concentration of which increases markedly within hours of any tissue-damaging, inflammatory event. However, upon dissociation of its pentameric quaternary structure, CRP subu...

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Bibliographic Details
Published in:The American journal of the medical sciences 2000-02, Vol.319 (2), p.79-83
Main Authors: Diehl, Edward E., Haines, G. Kenneth, Radosevich, James A., Potempa, Lawrence A.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal
Antigens - analysis
Arteries - chemistry
Biological and medical sciences
Blood vessel
C-reactive protein (CRP)
C-Reactive Protein - analysis
C-Reactive Protein - immunology
Endocardium - chemistry
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Humans
Immunohistochemistry
Intima
Lung - chemistry
Modified C-reactive protein (mCRP)
Proteins
Tunica Intima - chemistry
Tunica Media - chemistry
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Summary:Background: The prototypic acute phase reactant, C-reactive protein (CRP), is a serum soluble, cyclic pentameric protein, the concentration of which increases markedly within hours of any tissue-damaging, inflammatory event. However, upon dissociation of its pentameric quaternary structure, CRP subunits undergo a spontaneous and irreversible conformational change. The resulting molecule, termed modified CRP or mCRP, has reduced aqueous solubility and a propensity to aggregate into a matrix-like lattice structure. Methods: Using monoclonal antibodies, normal human tissues were immunohistochemically screened for the presence of CRP as well as mCRP antigens. Results: Significant levels of mCRP were detected in the walls of blood vessels associated with normal human tissues. These data indicate that mCRP is a naturally occurring form of CRP and that it is a tissue-based rather than serum-based molecule. Significance: This report describes the localization of a stable form of CRP, mCRP, in blood vessels associated with normal human tissues.
ISSN:0002-9629
1538-2990
DOI:10.1016/S0002-9629(15)40692-5