Method to produce 9(S)‐hydroperoxides of linoleic and linolenic acids by maize lipoxygenase

Seed from maize (corn) Zea mays provides a ready source of 9‐lipoxygenase that oxidizes linoleic acid and linolenic acid into 9(S)‐hydroperoxy‐10(F), 12(Z)‐octadecadienoic acid and 9(S)‐hydroperoxy‐10(E), 12(Z), 15(Z)‐octadecatrienoic acid, respectively. Corn seed has a very active hydro‐peroxide‐de...

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Bibliographic Details
Published in:Lipids 2001-05, Vol.36 (5), p.529-533
Main Authors: Gardner, Harold W., Grove, Marilyn J.
Format: Article
Language:English
Subjects:
alpha-Linolenic Acid - chemistry
alpha-Linolenic Acid - metabolism
Ammonium
Centrifugation
Chromatography, High Pressure Liquid
Corn
Fatty acids
Hydrogen-Ion Concentration
Linoleic Acid - chemistry
Linoleic Acid - metabolism
Lipid Peroxides - biosynthesis
Lipid Peroxides - chemistry
Lipoxygenase - metabolism
Seeds - enzymology
Stereoisomerism
Zea mays - enzymology
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Summary:Seed from maize (corn) Zea mays provides a ready source of 9‐lipoxygenase that oxidizes linoleic acid and linolenic acid into 9(S)‐hydroperoxy‐10(F), 12(Z)‐octadecadienoic acid and 9(S)‐hydroperoxy‐10(E), 12(Z), 15(Z)‐octadecatrienoic acid, respectively. Corn seed has a very active hydro‐peroxide‐decomposing enzyme, allene oxide synthase (AOS), which must be removed prior to oxidizing the fatty acid. A simple pH 4.5 treatment followed by centrifugation removes most of the AOS activity. Subsequent purification by ammonium sulfate fractional precipitation results in negligible improvement in 9‐hydroperoxide formation. This facile alternative method of preparing 9‐hydroperoxides has advantages over other commonly used plant lipoxygenases.
ISSN:0024-4201
1558-9307
DOI:10.1007/s11745-001-0753-1